Attributions
-
PI's
- Jan Kok
- Dirk-Jan Scheffers
-
Instructors
- Andreas Milias
- Linda Dijkshoorn
- Agnieszka Wegrzyn
- Sergey Lunev
-
General Support
- Lisbeth Ravnkilde Kjolbye: for providing us with many contacts to get our project off the ground
- Thanks to Matthijs’ personnel pass for free coffee
- Sonja Billerbeck: advice about the iGEM competition in general
- Siger Holsappel: general support in logistics
- Ger Telkamp: for providing us with lab-equipment from the practicum pool
- Manon Dusseljee: for financial administrative support
- Hannah Dosenko: for helping us with our ....
- Winand Slingenbergh and Fablab Groningen: for helping us with our 3D print designs
-
Fundraising help and advice
- Chienes Metus: For aiding us in our financial planning and taking care of all of our bills
- Mareike Schmidt: For giving advice on organizing our fundraising barbecue
- Derk Bosker: For guidance in the crowd-funding page
-
Lab Support
- Tau Jang: for providing us with A. Thaliana for extracting the PAL2 and general lab advice
- Jeroen Nijland: for guiding us with CRISPR-Cas9 and providing us with an extra lab and protocols and giving access to HPLC system
- Arnold Driessen: for advice about the use of CRISPR-Cas9 and evolution experiments in our project and lab access
- Jan Kiel: for helping us setting up our lab and providing us with cellulose
- Wiktor Szymanski: for advice about phosphorylation of cellulose and for allowing us to use space and materials in their laboratory
- Dirk Jan Slotboom: for providing us with access to a plate reader for the Interlab study
- Aditya Iyer: for help in the Interlab plate-reader experiments and supply of lab materials
- Hemant Kumar: for guidance in the cellulase activity assays & advise how to improve the HPLC protocol for the Bordeaux collaboration
- Alisa Garaeva: for providing us with a plasmid for our collaboration with iGEM Leiden
- Amanda van Tilburg: for delivery of primers and donation of BL21(DE3) strain
- Buu Minh Tran: for providing us with a plasmid for our collaboration with iGEM Leiden
- Vakhil Takhaveev: for being so kind to already transform these plasmids in E. coli for us
- Jakob Viel: for helping us find -80 freezer space, and locating some chemicals
- Marisya Majsnerowska: for providing us with materials to visualise our His-tagged proteins on Western blots
- Andre Boltjes: for feedback on the styrene extraction protocol
- Ruben Maaskant: for helping with the GC-MS and GC-FID methods
- Arjen Krikken: for providing strains, plasmids, and some restriction enzymes
- Huala Wu: for providing YPD Zeocin plates
- Anna Lauxen: for instructions on the Tecan Geneos platereader
-
Lab design Support
- Bert Poolman: for giving us advice on how to export styrene from the cell
- Jan Willem Meints: for helping us develop a HPLC quantification method for styrene
- Marco Fraaije: for advice about a cellulase-activity assay
- Dick Janssen: For advice on experimental design of the enzymatic cascade
-
Personal development and coaching
-
(Integrated) Human Practices and Public Engagement Support
- Karin Ree: for giving us tips on who to contact
- Tjerk Douwma: for helping us doing carbon footprint analysis (Life Cycle Analysis)
- Katja Loos: for advice on biodegradable plastics
- Francesco Picchioni: for advise on what plastic to go for
- Martin van Dord: for advice about plastics and rubbers (NRK)
- Pieter Imhof: for advice about the production of biochemicals (BioBTX)
- Machiel van Steenis (Program manager Innovative Biomass Applications): for advice about the application of biomass-based technologies
- Gert-Jan Euverink: for advice about the use of sewage material as a source of our cellulose and technical improvements for our yeast strain
- Frans Timmermans: for helping us extrapolate our project to alternative biomass sources (algae as cellulose source)
- Andre Fay: for sharing his expertise in the field of biomass
- Korienke Smit and Niek Savelkoul: for coaching us about integrated human practices (RIVM)
- ScienceLinx: for helping us with education and public engagement
- Scholierenacademie: for giving us the opportunity to educate primary school students about … Jens knows this
- Paddepoel: for giving us a stage for our outreach event to educate the general population on science and our project in general
- Girbe Buist: for supplying us with materials for our outreach event in Paddepoel
- Rene Fransen: for …
- Jeroen Tieleman (Bioclear Earth): for giving us tips on the resource of biomass
-
Outreach and Social Media
- Daniel Oostenbrink: for helping us fine-tune our animation’s script
- Joris-Jan Coenraads: for lending us his microphone to record our animation
-
Drylab Support
- Agnieszka Wegrzyn: for general modelling advice and advice on flux based modelling
- Matthias Heinemann: for giving us advice on modelling our project using Flux balance Analysis
- Siewert-Jan Marrink: for discussing modelling approaches with us
- Pim Frederix: for giving us a group workshop on Molecular Dynamics
- Paolo Telles de Souza: for helping to setup the cellulose binding molecular dynamics model