Workflow
KO株作成
Based on the Aache2017 result, we created KO yeast strains. the Best Basic part (BBa_K2403000).
How Rev worksPlasmid construction
Rev recruits CRM1, a principal nuclear export factor in cells which mediates export of cellular RNAs including snRNA and all 4 ribosomal RNAs to the cytoplasm.
Key advantage
Rev protein makes it possible to export complex RNA structures from the nucleus to the cytoplasm, which otherwise cannot be achieved effectively.