Team:Kyoto/Workflow

Workflow

KO株作成

Based on the Aache2017 result, we created KO yeast strains. the Best Basic part (BBa_K2403000).

How Rev worksPlasmid construction

Rev recruits CRM1, a principal nuclear export factor in cells which mediates export of cellular RNAs including snRNA and all 4 ribosomal RNAs to the cytoplasm.

Key advantage

Rev protein makes it possible to export complex RNA structures from the nucleus to the cytoplasm, which otherwise cannot be achieved effectively.