Team:SKLMT-China/Material

SKLMT-China

This is SKLMT-China test word
iGEM team from sdu test banner
wiki Here try long sentences

Description

QIAquick Gel Extraction Kit (Qiagen, cat. no. 28704) QIAquick PCR Purification Kit (Qiagen, cat. no. 28104) Qiagen Plasmid Mini Kit (Qiagen, cat. no. 12123) Tryptone (Oxoid, cat. no. LP0042) Yeast extract (Oxoid, cat. no. LP0021) NaCl (Sangon Biotec, cat. no. SB0476) Agar (Solarbio, cat. no. A8190) NaOH (Sigma-Aldrich, cat. no. S8045) Autoclaved ddH 2O kept at room temperature (RT, 18–22 °C) and on ice Tetracycline hydrochloride (Sigma-Aldrich, cat. no. T3383) Chloramphenicol (Sigma-Aldrich, cat. no. C1919) Ampicillin sodium salt (Sigma-Aldrich, cat. no. A9518) Kanamycin sulfate (Sigma-Aldrich, cat. no. K4000) Apramycin sulfate salt (Sigma-Aldrich, cat. no. A2024) Gentamicin solution (50 mg ml − 1; Sigma-Aldrich, cat. no. G1397) Hygromycin B (Invitrogen, cat. no. 10687-010) Streptomycin sulfate salt (Sigma-Aldrich, cat. no. A6501) Sodium acetate trihydrate (NaOAc•3H2O; Sinopharm, cat. no. 10018718) Lysozyme (Sigma-Aldrich, cat. no. 62970) Proteinase K (Roche, cat. no. 03115879001) Trizma base (Sigma-Aldrich, cat. no. T6066) Na2EDTA (Sigma-Aldrich, cat. no. E5134) HCl (Sinopharm, cat. no. 10011008) Sodium dodecyl sulfate (SDS; Sigma-Aldrich, cat. no. L3771) Phenol–chloroform–isoamyl alcohol mixture (25:24:1; Sigma-Aldrich,cat. no. 77617) Isopropanol (Sinopharm, cat. no. 80109218) Ethanol, absolute (Sinopharm, cat. no. 10009218) 70% (vol/vol) ethanol RNase A (10 mg ml − 1; DNase and protease free; Thermo Scientific,cat. no. EN0531) l-Arabinose (Sigma-Aldrich, cat. no. A3256) l-Rhamnose (Sigma-Aldrich, cat. no. 83650) 1-kb DNA ladder (New England BioLabs, cat. no. N3232) Restriction enzymes (New England BioLabs) PrimeSTAR Max DNA Polymerase (Takara, cat. no. R045B) 10× Tris/boric acid/EDTA (TBE) buffer (Bio-Rad, cat. no. 161-070) Ethidium bromide solution (10 mg/ml; Dingguo, cat. no. NEP028-1) Agarose (Takara, cat. no. 5261) Buffer P1 (Qiagen, cat. no. 19051) Buffer P2 (Qiagen, cat. no. 19052) Buffer P3 (Qiagen, cat. no. 19053)