Attributions
We could not have come this far without the aid of others. Thank you to everyone who believed in our project, and shared your knowledge and time with us.
Inspiration
Our project was inspired by the works of iGEM Peking 2015. We decided to improve on this, exchanging the split reporter enzyme with beta-lactamase. When cleaving the substrate nitrocefine, it produces a change in colour visible to the naked eye. Our decision to apply this to detecting vulvovaginal Candida albicans stemmed from the public lack of knowledge concerning the subject, and increasing antimycotic resistance.
Instructors
Without whom this project would not have been possible
DIRK LINKE Primary Instructor, course leader, and head of the Linke Group
KIRSTEN B. HARALDSEN Advisor and project support. Helped us with outreach and human practises.
ATHANASIOS SARAGLIADIS Instructor, provided us with invaluable help and advice during our labwork. We are forever grateful for your help.
Lab work
Thank you for your aid in the lab, and for your help with matters both practical and theoretical.
Marcella O. Rydmark Assisted us with difficult techniques, and protein purification
Ragnhild Eskeland Provided us with expert feedback on CRISPR and guide RNA
Sandra Lopez-Aviles Provided us with the yeast cells necessary for a proof-of-concept
National Reference Laboratory for Medical Mycology Many thanks to Cecilie T. Andersen and Lonny M. Kløvfjell for giving us a tour of the laboratory, and general advice concerning yeasts
Johannes Landskron Provided us with access to a flowcytometer, and helped us set it up for the Interlab study
Beata Nadratowska-Wesolowska Provided us with expert feedback on CRISPR and guide RNA
Bård Enger Mathiesen Technical lab assistance, especially with freeze-drying techniques
The Linke Group Thank you for your patience and warm welcome to the labspace
Human Practices
Thank you for helping us reach out to the community
Åslaug Brynhildsen Aided us with public outreach, and how to communicate with media
Elina Melteig Aided us with public outreach, and how to communicate with media
Project Support
Thank you for your assistance with matters small and large, and for keeping us in motion
Steinar Mortensen Helped us with booking flight and accomodation, as well as shipping items for sequencing
Ana Sifuentes When we had trouble with the Wiki, Ana was there to aid us and answer our questions
Katrine Kjenne Design our logo for us
Abigail Sison Our point-of-contact with HQ.
Bruno A. Dzogovic Helped is with the initial process of the Wiki
Heikki Sørum Greatly assisted us with making 3D-structures of our enzymes, and helped develop our prototype
Presentation feedback
Thank you for your honest feedback and great advice.
Anne Greulich Provided us with feedback and advice on how to improve our presentation
Thomas Trunk Helped improving our presentation
Yuri van Ekelenburg Helped improving our presentation
Miscellaneous
w3schools w3schools.com is a free resource for tutorials and a reference guide for web development. We wrote our wiki by ourselves, and this free resource helped us a lot.
Skeleton Skeleton is an open-source boilerplate written by Dave Gamache. It has helped us maintain the typography and scaling of elements on our website. With only ~400 lines of code, it's light weight makes it perfect for smaller projects like this. You can find more information at getskeleton.com
THANK YOU!