Team:TecCEM/InterLab

Synthetic biology and all the engineering disciplines need to be reliable and repeatable, this year iGEM TecCEM 2018 is one of the teams that participated in the Fifth International InterLaboratory Measurement Study. In this page, we report the results obtained during all protocols.

Competent cell - transformation DH5-alpha

Transformations of all devices were performed, and the resulting transformant strains were plated on LB agar, with 35 μg/mL chloramphenicol. Two colonies per plate were separated into two independent cultures.

Verification

We verified the presence and size of the device plasmids in the transformant strains by running an agarose gel electrophoresis.

Table 1. Devices from the Interlab Study with their pait number and respective size.

Device	Part Number	Size (bp)
Negative Control	BBa_R0040	2124
Positive Control	BBa_I20270	2989
Device 1	BBa_J364000	2988
Device 2	BBa_J364001	2988
Device 3	BBa_J364002	2988
Device 4	BBa_J364007	2988
Device 5	BBa_J364008	2988
Device 6	BBa_J364009	2988