Team:TecCEM/InterLab
Synthetic biology and all the engineering disciplines need to be reliable and repeatable, this year iGEM TecCEM 2018 is one of the teams that participated in the Fifth International InterLaboratory Measurement Study. In this page, we report the results obtained during all protocols.
Competent cell - transformation DH5-alpha
Transformations of all devices were performed, and the resulting transformant strains were plated on LB agar, with 35 μg/mL chloramphenicol. Two colonies per plate were separated into two independent cultures.
Verification
We verified the presence and size of the device plasmids in the transformant strains by running an agarose gel electrophoresis.
| Device | Part Number | Size (bp) |
|---|---|---|
| Negative Control | BBa_R0040 | 2124 |
| Positive Control | BBa_I20270 | 2989 |
| Device 1 | BBa_J364000 | 2988 |
| Device 2 | BBa_J364001 | 2988 |
| Device 3 | BBa_J364002 | 2988 |
| Device 4 | BBa_J364007 | 2988 |
| Device 5 | BBa_J364008 | 2988 |
| Device 6 | BBa_J364009 | 2988 |
Calibration Protocol
The main objective of this calibration LUDOX protocol is to use LUDOX CL-X to get the conversion factor between absorbance (Abs600) from the plate reader and the OD600 measurement.
| LUDOX CL-X | H2O | |
|---|---|---|
| Replicate 1 | 0.059 | 0.033 |
| Replicate 2 | 0.061 | 0.033 |
| Replicate 3 | 0.062 | 0.033 |
| Replicate 4 | 0.060 | 0.033 |
| Arith. Mean | 0.061 | 0.033 |
| Corrected Abs600 | 0.028 | |
| Reference OD600 | 0.063 | |
| OD600/Abs600 | 2.291 |