InterLab
We did this year's iGEM InterLab study!
Our original Excel file can be downloaded <a href="https://2018.igem.org/Team:Virginia/InterLab_2018_Virginia.iGEM.xlsx" download="">here</a>.
<a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf">Here</a> is the protocol that we followed.
For more information, refer to this <a href="https://docs.google.com/document/d/1HzD6SxlGUwsEZjJ4m1vHhFBHEeCzOM1v-d8jamopM1k/edit?usp=sharing" target="_blank">Google doc</a>.
OD600 Reference Point - LUDOX Protocol
|
LUDOX CL-X |
H2O |
| Replicate 1 |
0.113 |
0.087 |
| Replicate 2 |
0.105 |
0.085 |
| Replicate 3 |
0.119 |
0.084 |
| Replicate 4 |
0.103 |
0.085 |
| Arith. Mean |
0.110 |
0.085 |
| Corrected Abs600 |
0.025 |
|
| Reference OD600 |
0.063 |
|
| OD600/Abs600 |
2.545 |
|
Particle Standard Curve: Microsphere Protocol
Need to color code but:
Abs600 measurements are blue cells.
Gold cells are calculated.
| Number of Particles |
2.35E+08 |
1.18E+08 |
5.88E+07 |
2.94E+07 |
1.47E+07 |
7.35E+06 |
3.68E+06 |
1.84E+06 |
9.19E+05 |
4.60E+05 |
2.30E+05 |
0 |
| Replicate 1 |
0.534 |
0.337 |
0.211 |
0.148 |
0.124 |
0.102 |
0.094 |
0.091 |
0.089 |
0.089 |
0.088 |
0.087 |
| Replicate 2 |
0.538 |
0.329 |
0.204 |
0.145 |
0.117 |
0.102 |
0.099 |
0.092 |
0.089 |
0.089 |
0.086 |
0.085 |
| Replicate 3 |
0.543 |
0.332 |
0.209 |
0.145 |
0.116 |
0.107 |
0.093 |
0.090 |
0.088 |
0.089 |
0.086 |
0.083 |
| Replicate 4 |
0.545 |
0.314 |
0.208 |
0.143 |
0.119 |
0.102 |
0.092 |
0.089 |
0.089 |
0.089 |
0.086 |
0.083 |
| Arith. Mean |
0.540 |
0.328 |
0.208 |
0.145 |
0.119 |
0.103 |
0.095 |
0.091 |
0.089 |
0.089 |
0.087 |
0.085 |
| Arith. Std.Dev. |
0.005 |
0.010 |
0.003 |
0.002 |
0.004 |
0.003 |
0.003 |
0.001 |
0.001 |
0.000 |
0.001 |
0.002 |
| Arith. Net Mean |
0.456 |
0.244 |
0.124 |
0.061 |
0.035 |
0.019 |
0.010 |
0.006 |
0.004 |
0.004 |
0.002 |
|
<img src="
" alt="ParticleStandardCurve">
<img src="
" alt="ParticleStandardCurve_log">
Particles/OD
Note: Final scaling level determined from medium-high points likely to be less impacted by saturation or pipetting error. If needed, you can shift which points are used, but it is likely better to correct instrument settings and protocol.
| Number of Particles |
2.35E+08 |
1.18E+08 |
5.88E+07 |
2.94E+07 |
1.47E+07 |
7.35E+06 |
3.68E+06 |
1.84E+06 |
9.19E+05 |
4.60E+05 |
2.30E+05 |
| Mean particles / Abs600 |
5.17E+08 |
4.83E+08 |
4.76E+08 |
4.84E+08 |
4.26E+08 |
3.92E+08 |
3.68E+08 |
3.06E+08 |
2.16E+08 |
1.02E+08 |
1.15E+08 |
| Mean of med-high levels: |
|
4.52E+08 |
|
|
|
|
|
|
|
|
|
Cospheric Monodisperse Silica Microspheres 0.961um diameter
| Spheres/gram |
Grams/mL |
Spheres/0.55mL |
Resuspend volume mL: |
Dilution X: |
Total volume mL: |
Particles/mL: |
Well volume (mL) |
Initial particles: |
| 1.20E+12 |
1.8 |
1.19E+12 |
2.55 |
100 |
255 |
4.71E+09 |
0.1 |
4.71E+08 |
Raw Plate Reader Measurements
Fluorescence Raw Readings:
| Hour 0: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
LB + Chlor (blank) |
| Colony 1, Replicate 1 |
124.47 |
172.15 |
136.79 |
137.65 |
115.66 |
132.36 |
127.35 |
135.34 |
112.83 |
| Colony 1, Replicate 2 |
121.01 |
142.23 |
113.72 |
130.07 |
93.041 |
106.03 |
104.61 |
113.2 |
98.307 |
| Colony 1, Replicate 3 |
123.38 |
148.37 |
112.73 |
131.37 |
90.708 |
106.35 |
104.49 |
113.95 |
95.141 |
| Colony 1, Replicate 4 |
119.97 |
141.57 |
113.49 |
124.8 |
93.35 |
107.29 |
102.57 |
111.37 |
96.012 |
| Colony 2, Replicate 1 |
121.32 |
134.7 |
116.21 |
169.04 |
93.865 |
110.22 |
152.05 |
111.67 |
99.513 |
| Colony 2, Replicate 2 |
134.51 |
141.18 |
115.5 |
131.01 |
94.94 |
107.69 |
124.91 |
114.24 |
96.457 |
| Colony 2, Replicate 3 |
130.47 |
141.41 |
114.54 |
130.7 |
92.262 |
111.95 |
128.96 |
115.03 |
98.671 |
| Colony 2, Replicate 4 |
129.77 |
160.97 |
142.19 |
130.7 |
112.12 |
128.64 |
155.08 |
129.88 |
122.04 |
| Hour 6: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
LB + Chlor (blank) |
| Colony 1, Replicate 1 |
147.58 |
923.47 |
481.57 |
768.79 |
145.84 |
158.29 |
196.74 |
472.33 |
130.21 |
| Colony 1, Replicate 2 |
135.57 |
868.37 |
443.8 |
771.52 |
133.54 |
150.48 |
174.77 |
442.69 |
108.87 |
| Colony 1, Replicate 3 |
133.91 |
821.45 |
435.09 |
749.58 |
135.07 |
147.14 |
176.84 |
433.8 |
106.07 |
| Colony 1, Replicate 4 |
131.16 |
825.34 |
424.75 |
702.31 |
136.56 |
145.48 |
178.85 |
435.43 |
101.34 |
| Colony 2, Replicate 1 |
129.25 |
736.73 |
506.04 |
721.65 |
130.79 |
152.77 |
263.05 |
404.78 |
105.13 |
| Colony 2, Replicate 2 |
129.93 |
746.95 |
522.99 |
752.02 |
130.12 |
154.08 |
274.44 |
99.78 |
102.2 |
| Colony 2, Replicate 3 |
134.9 |
737.42 |
519.61 |
760.98 |
129.32 |
162.53 |
277.69 |
390.5 |
104.1 |
| Colony 2, Replicate 4 |
136.44 |
824.81 |
585.7 |
749.59 |
145.38 |
171.03 |
319.39 |
429.11 |
122.55 |
Abs600 Raw Readings
| Hour 0: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
LB + Chlor (blank) |
| Colony 1, Replicate 1 |
0.114 |
0.112 |
0.112 |
0.116 |
0.115 |
0.113 |
0.11 |
0.112 |
0.097 |
| Colony 1, Replicate 2 |
0.113 |
0.112 |
0.108 |
0.108 |
0.109 |
0.109 |
0.106 |
0.112 |
0.097 |
| Colony 1, Replicate 3 |
0.114 |
0.11 |
0.106 |
0.108 |
0.109 |
0.108 |
0.106 |
0.111 |
0.093 |
| Colony 1, Replicate 4 |
0.114 |
0.11 |
0.106 |
0.108 |
0.109 |
0.107 |
0.105 |
0.109 |
0.095 |
| Colony 2, Replicate 1 |
0.115 |
0.114 |
0.108 |
0.123 |
0.111 |
0.112 |
0.124 |
0.114 |
0.094 |
| Colony 2, Replicate 2 |
0.115 |
0.112 |
0.107 |
0.108 |
0.109 |
0.109 |
0.11 |
0.111 |
0.096 |
| Colony 2, Replicate 3 |
0.114 |
0.111 |
0.107 |
0.107 |
0.11 |
0.111 |
0.116 |
0.111 |
0.093 |
| Colony 2, Replicate 4 |
0.115 |
0.113 |
0.109 |
0.11 |
0.11 |
0.109 |
0.11 |
0.11 |
0.095 |
| Hour 6: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
LB + Chlor (blank) |
| Colony 1, Replicate 1 |
0.585 |
0.526 |
0.49 |
0.538 |
0.594 |
0.545 |
0.558 |
0.595 |
0.096 |
| Colony 1, Replicate 2 |
0.563 |
0.533 |
0.483 |
0.533 |
0.582 |
0.544 |
0.541 |
0.56 |
0.093 |
| Colony 1, Replicate 3 |
0.595 |
0.538 |
0.498 |
0.525 |
0.585 |
0.566 |
0.54 |
0.531 |
0.094 |
| Colony 1, Replicate 4 |
0.586 |
0.532 |
0.49 |
0.494 |
0.581 |
0.571 |
0.5 |
0.569 |
0.094 |
| Colony 2, Replicate 1 |
0.548 |
0.543 |
0.502 |
0.527 |
0.553 |
0.565 |
0.544 |
0.564 |
0.094 |
| Colony 2, Replicate 2 |
0.566 |
0.543 |
0.482 |
0.536 |
0.544 |
0.544 |
0.526 |
0.541 |
0.096 |
| Colony 2, Replicate 3 |
0.528 |
0.514 |
0.484 |
0.535 |
0.55 |
0.519 |
0.507 |
0.561 |
0.093 |
| Colony 2, Replicate 4 |
0.52 |
0.516 |
0.462 |
0.496 |
0.53 |
0.523 |
0.488 |
0.524 |
0.097 |
Fluorescence per OD
uM Fluorescein / OD
| Hour 0: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
0.066 |
0.382 |
0.154 |
0.126 |
0.015 |
0.118 |
0.108 |
0.145 |
| Colony 1, Replicate 2 |
0.137 |
0.283 |
0.135 |
0.279 |
-0.042 |
0.062 |
0.068 |
0.096 |
| Colony 1, Replicate 3 |
0.130 |
0.302 |
0.131 |
0.233 |
-0.027 |
0.072 |
0.069 |
0.101 |
| Colony 1, Replicate 4 |
0.122 |
0.293 |
0.153 |
0.214 |
-0.018 |
0.091 |
0.063 |
0.106 |
| Colony 2, Replicate 1 |
0.100 |
0.170 |
0.115 |
0.231 |
-0.032 |
0.057 |
0.169 |
0.059 |
| Colony 2, Replicate 2 |
0.193 |
0.270 |
0.167 |
0.278 |
-0.011 |
0.083 |
0.196 |
0.114 |
| Colony 2, Replicate 3 |
0.146 |
0.229 |
0.109 |
0.221 |
-0.036 |
0.071 |
0.127 |
0.088 |
| Colony 2, Replicate 4 |
0.037 |
0.209 |
0.139 |
0.056 |
-0.064 |
0.046 |
0.213 |
0.050 |
| Hour 6: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
0.003 |
0.178 |
0.086 |
0.139 |
0.003 |
0.006 |
0.014 |
0.066 |
| Colony 1, Replicate 2 |
0.005 |
0.167 |
0.083 |
0.145 |
0.005 |
0.009 |
0.014 |
0.069 |
| Colony 1, Replicate 3 |
0.005 |
0.156 |
0.079 |
0.144 |
0.006 |
0.008 |
0.015 |
0.072 |
| Colony 1, Replicate 4 |
0.006 |
0.160 |
0.079 |
0.145 |
0.007 |
0.009 |
0.018 |
0.068 |
| Colony 2, Replicate 1 |
0.005 |
0.136 |
0.095 |
0.137 |
0.005 |
0.010 |
0.034 |
0.062 |
| Colony 2, Replicate 2 |
0.006 |
0.139 |
0.105 |
0.143 |
0.006 |
0.011 |
0.039 |
0.065 |
| Colony 2, Replicate 3 |
0.007 |
0.145 |
0.103 |
0.143 |
0.005 |
0.013 |
0.040 |
0.059 |
| Colony 2, Replicate 4 |
0.003 |
0.162 |
0.123 |
0.152 |
0.005 |
0.011 |
0.049 |
0.069 |
Net Fluorescein a.u.
| Hour 0: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
11.64 |
59.32 |
23.96 |
24.82 |
2.83 |
19.53 |
14.52 |
22.51 |
| Colony 1, Replicate 2 |
22.70 |
43.92 |
15.41 |
31.76 |
-5.27 |
7.72 |
6.30 |
14.89 |
| Colony 1, Replicate 3 |
28.24 |
53.23 |
17.59 |
36.23 |
-4.43 |
11.21 |
9.35 |
18.81 |
| Colony 1, Replicate 4 |
23.96 |
45.56 |
17.48 |
28.79 |
-2.66 |
11.28 |
6.56 |
15.36 |
| Colony 2, Replicate 1 |
21.81 |
35.19 |
16.70 |
69.53 |
-5.65 |
10.71 |
52.54 |
12.16 |
| Colony 2, Replicate 2 |
38.05 |
44.72 |
19.04 |
34.55 |
-1.52 |
11.23 |
28.45 |
17.78 |
| Colony 2, Replicate 3 |
31.80 |
42.74 |
15.87 |
32.03 |
-6.41 |
13.28 |
30.29 |
16.36 |
| Colony 2, Replicate 4 |
7.73 |
38.93 |
20.15 |
8.66 |
-9.92 |
6.60 |
33.04 |
7.84 |
| Hour 6: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
17.37 |
793.26 |
351.36 |
638.58 |
15.63 |
28.08 |
66.53 |
342.12 |
| Colony 1, Replicate 2 |
26.70 |
759.50 |
334.93 |
662.65 |
24.67 |
41.61 |
65.90 |
333.82 |
| Colony 1, Replicate 3 |
27.84 |
715.38 |
329.02 |
643.51 |
29.00 |
41.07 |
70.77 |
327.73 |
| Colony 1, Replicate 4 |
29.82 |
724.00 |
323.41 |
600.97 |
35.22 |
44.14 |
77.51 |
334.09 |
| Colony 2, Replicate 1 |
24.12 |
631.60 |
400.91 |
616.52 |
25.66 |
47.64 |
157.92 |
299.65 |
| Colony 2, Replicate 2 |
27.73 |
644.75 |
420.79 |
649.82 |
27.92 |
51.88 |
172.24 |
297.58 |
| Colony 2, Replicate 3 |
30.80 |
633.32 |
415.51 |
656.88 |
25.22 |
58.43 |
173.59 |
286.40 |
| Colony 2, Replicate 4 |
13.89 |
702.26 |
463.15 |
627.04 |
22.83 |
48.48 |
196.84 |
306.56 |
Net Abs 600
| Hour 0: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
0.017 |
0.015 |
0.015 |
0.019 |
0.018 |
0.016 |
0.013 |
0.015 |
| Colony 1, Replicate 2 |
0.016 |
0.015 |
0.011 |
0.011 |
0.012 |
0.012 |
0.009 |
0.015 |
| Colony 1, Replicate 3 |
0.021 |
0.017 |
0.013 |
0.015 |
0.016 |
0.015 |
0.013 |
0.018 |
| Colony 1, Replicate 4 |
0.019 |
0.015 |
0.011 |
0.013 |
0.014 |
0.012 |
0.010 |
0.014 |
| Colony 2, Replicate 1 |
0.021 |
0.020 |
0.014 |
0.029 |
0.017 |
0.018 |
0.030 |
0.020 |
| Colony 2, Replicate 2 |
0.019 |
0.016 |
0.011 |
0.012 |
0.013 |
0.013 |
0.014 |
0.015 |
| Colony 2, Replicate 3 |
0.021 |
0.018 |
0.014 |
0.014 |
0.017 |
0.018 |
0.023 |
0.018 |
| Colony 2, Replicate 4 |
0.020 |
0.018 |
0.014 |
0.015 |
0.015 |
0.014 |
0.015 |
0.015 |
| Hour 6: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
0.489 |
0.430 |
0.394 |
0.442 |
0.498 |
0.449 |
0.462 |
0.499 |
| Colony 1, Replicate 2 |
0.470 |
0.440 |
0.390 |
0.440 |
0.489 |
0.451 |
0.448 |
0.467 |
| Colony 1, Replicate 3 |
0.501 |
0.444 |
0.404 |
0.431 |
0.491 |
0.472 |
0.446 |
0.437 |
| Colony 1, Replicate 4 |
0.492 |
0.438 |
0.396 |
0.400 |
0.487 |
0.477 |
0.406 |
0.475 |
| Colony 2, Replicate 1 |
0.454 |
0.449 |
0.408 |
0.433 |
0.459 |
0.471 |
0.450 |
0.470 |
| Colony 2, Replicate 2 |
0.470 |
0.447 |
0.386 |
0.440 |
0.448 |
0.448 |
0.430 |
0.445 |
| Colony 2, Replicate 3 |
0.435 |
0.421 |
0.391 |
0.442 |
0.457 |
0.426 |
0.414 |
0.468 |
| Colony 2, Replicate 4 |
0.423 |
0.419 |
0.365 |
0.399 |
0.433 |
0.426 |
0.391 |
0.427 |
Fluorescence per Particle
MEFL / particle
| Hour 0: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
2.24E+03 |
1.29E+04 |
5.23E+03 |
4.27E+03 |
5.14E+02 |
3.99E+03 |
3.65E+03 |
4.91E+03 |
| Colony 1, Replicate 2 |
4.64E+03 |
9.58E+03 |
4.58E+03 |
9.45E+03 |
-1.44E+03 |
2.11E+03 |
2.29E+03 |
3.25E+03 |
| Colony 1, Replicate 3 |
4.40E+03 |
1.02E+04 |
4.43E+03 |
7.90E+03 |
-9.06E+02 |
2.44E+03 |
2.35E+03 |
3.42E+03 |
| Colony 1, Replicate 4 |
4.12E+03 |
9.94E+03 |
5.20E+03 |
7.24E+03 |
-6.22E+02 |
3.07E+03 |
2.15E+03 |
3.59E+03 |
| Colony 2, Replicate 1 |
3.40E+03 |
5.76E+03 |
3.90E+03 |
7.84E+03 |
-1.09E+03 |
1.95E+03 |
5.73E+03 |
1.99E+03 |
| Colony 2, Replicate 2 |
6.55E+03 |
9.14E+03 |
5.66E+03 |
9.42E+03 |
-3.82E+02 |
2.83E+03 |
6.65E+03 |
3.88E+03 |
| Colony 2, Replicate 3 |
4.95E+03 |
7.77E+03 |
3.71E+03 |
7.48E+03 |
-1.23E+03 |
2.41E+03 |
4.31E+03 |
2.97E+03 |
| Colony 2, Replicate 4 |
1.26E+03 |
7.08E+03 |
4.71E+03 |
1.89E+03 |
-2.16E+03 |
1.54E+03 |
7.21E+03 |
1.71E+03 |
| Hour 6: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
1.16E+02 |
6.03E+03 |
2.92E+03 |
4.73E+03 |
1.03E+02 |
2.05E+02 |
4.71E+02 |
2.24E+03 |
| Colony 1, Replicate 2 |
1.86E+02 |
5.65E+03 |
2.81E+03 |
4.93E+03 |
1.65E+02 |
3.02E+02 |
4.81E+02 |
2.34E+03 |
| Colony 1, Replicate 3 |
1.82E+02 |
5.27E+03 |
2.66E+03 |
4.88E+03 |
1.93E+02 |
2.85E+02 |
5.19E+02 |
2.45E+03 |
| Colony 1, Replicate 4 |
1.98E+02 |
5.41E+03 |
2.67E+03 |
4.91E+03 |
2.37E+02 |
3.03E+02 |
6.25E+02 |
2.30E+03 |
| Colony 2, Replicate 1 |
1.74E+02 |
4.60E+03 |
3.21E+03 |
4.66E+03 |
1.83E+02 |
3.31E+02 |
1.15E+03 |
2.09E+03 |
| Colony 2, Replicate 2 |
1.93E+02 |
4.72E+03 |
3.57E+03 |
4.83E+03 |
2.04E+02 |
3.79E+02 |
1.31E+03 |
2.19E+03 |
| Colony 2, Replicate 3 |
2.32E+02 |
4.92E+03 |
3.48E+03 |
4.86E+03 |
1.81E+02 |
4.49E+02 |
1.37E+03 |
2.00E+03 |
| Colony 2, Replicate 4 |
1.07E+02 |
5.48E+03 |
4.15E+03 |
5.14E+03 |
1.72E+02 |
3.72E+02 |
1.65E+03 |
2.35E+03 |
Net Fluorescein a.u.
| Hour 0: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
11.64 |
59.32 |
23.96 |
24.82 |
2.83 |
19.53 |
14.52 |
22.51 |
| Colony 1, Replicate 2 |
22.70 |
43.92 |
15.41 |
31.76 |
-5.27 |
7.72 |
6.30 |
14.89 |
| Colony 1, Replicate 3 |
28.24 |
53.23 |
17.59 |
36.23 |
-4.43 |
11.21 |
9.35 |
18.81 |
| Colony 1, Replicate 4 |
23.96 |
45.56 |
17.48 |
28.79 |
-2.66 |
11.28 |
6.56 |
15.36 |
| Colony 2, Replicate 1 |
21.81 |
35.19 |
16.70 |
69.53 |
-5.65 |
10.71 |
52.54 |
12.16 |
| Colony 2, Replicate 2 |
38.05 |
44.72 |
19.04 |
34.55 |
-1.52 |
11.23 |
28.45 |
17.78 |
| Colony 2, Replicate 3 |
31.80 |
42.74 |
15.87 |
32.03 |
-6.41 |
13.28 |
30.29 |
16.36 |
| Colony 2, Replicate 4 |
7.73 |
38.93 |
20.15 |
8.66 |
-9.92 |
6.60 |
33.04 |
7.84 |
| Hour 6: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
17.37 |
793.26 |
351.36 |
638.58 |
15.63 |
28.08 |
66.53 |
342.12 |
| Colony 1, Replicate 2 |
26.70 |
759.50 |
334.93 |
662.65 |
24.67 |
41.61 |
65.90 |
333.82 |
| Colony 1, Replicate 3 |
27.84 |
715.38 |
329.02 |
643.51 |
29.00 |
41.07 |
70.77 |
327.73 |
| Colony 1, Replicate 4 |
29.82 |
724.00 |
323.41 |
600.97 |
35.22 |
44.14 |
77.51 |
334.09 |
| Colony 2, Replicate 1 |
24.12 |
631.60 |
400.91 |
616.52 |
25.66 |
47.64 |
157.92 |
299.65 |
| Colony 2, Replicate 2 |
27.73 |
644.75 |
420.79 |
649.82 |
27.92 |
51.88 |
172.24 |
297.58 |
| Colony 2, Replicate 3 |
30.80 |
633.32 |
415.51 |
656.88 |
25.22 |
58.43 |
173.59 |
286.40 |
| Colony 2, Replicate 4 |
13.89 |
702.26 |
463.15 |
627.04 |
22.83 |
48.48 |
196.84 |
306.56 |
Net Abs 600
| Hour 0: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
0.017 |
0.015 |
0.015 |
0.019 |
0.018 |
0.016 |
0.013 |
0.015 |
| Colony 1, Replicate 2 |
0.016 |
0.015 |
0.011 |
0.011 |
0.012 |
0.012 |
0.009 |
0.015 |
| Colony 1, Replicate 3 |
0.021 |
0.017 |
0.013 |
0.015 |
0.016 |
0.015 |
0.013 |
0.018 |
| Colony 1, Replicate 4 |
0.019 |
0.015 |
0.011 |
0.013 |
0.014 |
0.012 |
0.010 |
0.014 |
| Colony 2, Replicate 1 |
0.021 |
0.020 |
0.014 |
0.029 |
0.017 |
0.018 |
0.030 |
0.020 |
| Colony 2, Replicate 2 |
0.019 |
0.016 |
0.011 |
0.012 |
0.013 |
0.013 |
0.014 |
0.015 |
| Colony 2, Replicate 3 |
0.021 |
0.018 |
0.014 |
0.014 |
0.017 |
0.018 |
0.023 |
0.018 |
| Colony 2, Replicate 4 |
0.020 |
0.018 |
0.014 |
0.015 |
0.015 |
0.014 |
0.015 |
0.015 |
| Hour 6: |
Neg. Control |
Pos. Control |
Device 1 |
Device 2 |
Device 3 |
Device 4 |
Device 5 |
Device 6 |
| Colony 1, Replicate 1 |
0.489 |
0.430 |
0.394 |
0.442 |
0.498 |
0.449 |
0.462 |
0.499 |
| Colony 1, Replicate 2 |
0.470 |
0.440 |
0.390 |
0.440 |
0.489 |
0.451 |
0.448 |
0.467 |
| Colony 1, Replicate 3 |
0.501 |
0.444 |
0.404 |
0.431 |
0.491 |
0.472 |
0.446 |
0.437 |
| Colony 1, Replicate 4 |
0.492 |
0.438 |
0.396 |
0.400 |
0.487 |
0.477 |
0.406 |
0.475 |
| Colony 2, Replicate 1 |
0.454 |
0.449 |
0.408 |
0.433 |
0.459 |
0.471 |
0.450 |
0.470 |
| Colony 2, Replicate 2 |
0.470 |
0.447 |
0.386 |
0.440 |
0.448 |
0.448 |
0.430 |
0.445 |
| Colony 2, Replicate 3 |
0.435 |
0.421 |
0.391 |
0.442 |
0.457 |
0.426 |
0.414 |
0.468 |
| Colony 2, Replicate 4 |
0.423 |
0.419 |
0.365 |
0.399 |
0.433 |
0.426 |
0.391 |
0.427 |
