Team:SCU-China
Inspired by the modularization, call-and-return and do-not-reinvent-the-wheel philosophy in computer programming design, we come up with the idea of using the dCas9 protein to manipulate the expression of proteins and implement complex logic in a single E. coli cell this year. we want to construct versatile “library strains” that contains many, and maybe redundant coding sequences of commonly used proteins which are dormant by default; when we need to use the function of particular already coded proteins, we just simply transform a much smaller “Minimid” containing sgDNAs targeting the desired proteins into the library strain, and then utilizing the dCas9-sgRNA complex to initiate the transcription then expression of the particular protein.