Team:AHUT China/INTEGRATED FOR GOLD
1 . HP--Creative Art Course for primary school students
AHUT_China（This year's project is about carbon dioxide.）With the increasing impact of the greenhouse effect on the global environment,it’s very important to know how to efficiently deal with greenhouse gas CO2.Traditional CO2 collection technology is not mature, restricted by high energy consumption and low efficiency.
Our project simulates the protein molecule expressed by the mutation of carbonic anhydrase gene Loci, obtains the high thermal stability of carbonic anhydrase (CA-2) and imports it into Escherichia coli cells to achieve efficient CO2 collection
On July 5, 2018, we arrived at the XiangXiangle Art and Culture House, and we had a creative art class with the children.
In class, we studied biology with them and learned creative art courses.By letting children learn the signs of iGEM competition and explaining biological knowledge for them, especially the concepts of genes and synthetic biology, etc.,We encouraged children to add their own ideas to the iGEM logo and use painting to depict genes, synthetic biology, and iGEM competitions in their heart.As shown in Picture 3.
After learning the course we designed, every child completed their own works with the guidance of the teacher Long Lijuan and the iGEM team members.
Our Human Practice-- Creative Art Courses in Primary School Students is very meaningful. It not only introduces the iGEM competition, but also introduces the iGEM team of the Anhui University of Technology.And at the same time, this activity guides the children to have a preliminary understanding of life and the corresponding disciplines.
2 . We discussed with the AHUT's model airplane team
Currently, our project is still at the laboratory stage, has not yet reached large-scale application.To better seek the scene of large-scale applications, Anhui University of Technology's model airplane team and we held a seminar and developed a program of social practice with the help of the instructor group.
We got in touch with a factory in Maanshan(Maanshan Steel Plant), and a six-rotor drone was provided by the model team, as shown in Picture 1
Zhao Lei, who has studied embedded programming development in the team, has developed a carbon dioxide detection device that can be mounted on the unmanned aerial vehicle with different gas sensors (The gas sensor can detect the mass of carbon dioxide, sulfur dioxide and other gases in each liter.) and STM32 ARM microcontroller. As shown in Picture 3:
We used this drone to carry the detection device into the air and tested it near the end of the factory's exhaust system. It was found that the concentration of carbon dioxide near the smoke extraction device was extremely high, 5-20 times greater than the normal value. According to the document "National Greenhouse Gas Emissions Accounting and Reporting and Other 11 Greenhouse Gas Management National Standards Released", the proportion of various costs in the exhaust gas from such a plant's production process is: oxygen: carbon dioxide: sulfur dioxide: vulcanization Hydrogen: Carbon monoxide: Hydrogen chloride: Fluoride: Nitrogen oxides: Other = 14:10:3:3:3:2:3:8:54,
We have measured the composition, content and mass percentage of each gas in the factory exhaust gas after averaging as follows:
3. Biofilm Process Water Purification Laboratory
On September 8, 2018, our AHUT-China team visited the Biofilm Process Water Purification Laboratory of the College of Architecture and Engineering of Anhui University of Technology. The graduate student Xu Fan led us to visit the laboratory. The seniors introduced us to the principle of purifying sewage in the laboratory, which made us understand that relying on the microbial membrane fixed on the surface of the carrier to degrade organic matter has the characteristics of good effect and low technology and low cost.
Then we introduced our project to the seniors. The seniors said that many problems caused by CO2 also brought us many problems. Therefore, he has great hope for our project. At the same time, we have learned that the biomembrance process can not only treat sewage, but also can be applied to the treatment of organic gases. The pollutants in the organic waste gas are first contacted with water and dissolved in water, that is, diffused from the gas film into the liquid film; The organic pollutant components in the liquid film are diffused to the biofilm by the difference in concentration, and they are captured and absorbed by the microorganisms therein; the organic pollutants entering the microorganisms are decomposed as energy and nutrients in the metabolism of the microorganisms themselves. This provides us with some ideas on the combination of theory and practice.
Through a visit to the Biofilm Process Water Purification Laboratory in the afternoon, we learned about the biofilm treatment of sewage. At the same time, we also learned the necessity of our project. The impact of greenhouse gas CO2 cannot be underestimated. We also hope that our project can be applied to practice. The knowledge of biofilm processes in the treatment of organic waste gases has also given us some inspiration, and we may be able to use this knowledge when dealing with CO2.
5. Public number iGEM WeChat of Anhui University of Technology
AHUT_China upgraded the original WeChat public number in July 2018. During the implementation of this year's project, more than ten original articles have been published since the establishment of the team. We actively carry out social practice through such external publicity platform, popularize iGEM contest and introduce our project this year.
Here's part of the title of the original article:
1. Biofilm water purification laboratory (September 17, 2018)
2. Communication and cooperation -- (Sweden) iGEM (lingshou-sweden) of Linkoping University (September 12, 2018)
3. Bio-chemical research center ---- -professor Xu xia (September 11, 2018)
4. International cooperation - Harvard university iGEM team (Harvard) (4 September 2018)
5. International cooperation - IIT -madras team (30 August 2018)
5. Thumb up for iGEMer spirit (July 29, 2018)
7. Coli (July 26, 2018)
8. Carbonic acid bacteria (25 July 2018)
9. What is IGEM? (July 23, 2018)
After each HP activity, the members of AHUT_china group timely summarized, wrote the text, translated it into English, and made it into WeChat manuscript, which was published on the public account of iGEM WeChat of Anhui University of Technology.
Public account iGEM WeChat of the Anhui University of Technology, including three main sections of project progress, cooperation and communication, and popular science articles, for more convenient access and reading by subscribers.
The project progress module makes the team weekly publication, the experiment process and the team Logo display. The team weekly report is a periodic report, and the team often holds meetings, from 2018-03-10 weekly report to 2018-06-23 weekly report, a total of 11 pieces, recording how we prepare step by step, and continue to implement, to promote the process of the competition, to bring everyone into our laboratory, reveal all kinds of laboratory, and tell the hard but happy life of the co-generation.
Cooperation and communication are also divided into two parts. HP and Collaboration record some activities in HP, such as primary school students' creative class activities, visits to Huvernon environmental protection technology co, LTD, etc. Collaboration introduces our interaction with iGEM teams in other universities.
The popular science article module focuses on the popular science of synthetic biology. It mainly propagates the International genetic engineering Competition (iGEM) through words and pictures. It has been held by the Massachusetts Institute of Technology (MIT) every year since 2005. It is the highest international academic competition in the field of Synthetic Biology. And the knowledge related to our team's experiment, such as the popularization of carbonic anhydrase and escherichia coli.
The public account of iGEM WeChat of Anhui University of Technology is mainly for schools and the society. It can be promoted to WeChat users through WeChat channel, so as to improve the popularity of the international genetic engineering competition and create the image of the iGEM team of Anhui University of Technology.
11. Lecture on iGEM in Anhui University of Technology (AHUT_China) by Feiyue Lou
Time: 3.30p.m. on Sept. 11, 2018, Tuesday
Location: Conference Room in the Innovative Education Faculty
We held a lecture aiming at letting more people know about synthetic biology and iGEM. With great honor, we invited Dr. Pei hao, who is currently working in the school of engineering and applied sciences in Harvard University and engaging in the application of microfluidics in biomedical field, to give students in AHUT this lecture. It has successfully attracted interests of hundreds of students, but due to the limited seats in the meeting room, we had to invited only more than 60 students to attend this speech. In order to enable more people to watch the lecture, we took advantage of the webcast platform, making more than 1,000 people enjoy this meeting online. Dr. Pei not only presented us the research progress of microfluidics but also shared teachers and students with the current research results.
Microfluidics Technology refers to the technology of controlling, operating and detecting complex fluids at the micro size. It is a newly interdisciplinary subject developed on the basis of microelectronics, micromechanics, bioengineering and nanotechnology. The rapid development of Microfluidics Technology in recent years has stricken revolutionary impacts in the fields of chemistry, medicine and life science. Biochips are regarded as an important tool for interpretating gene sequences in the Post-Genome Era and microfluidics chips are also known as "lab-on-a-chip", which use MEMS (Micro Electro Mechanical Systems) technology for mixing the separated purification used in general laboratories and miniaturize devices such as enzyme reactions onto the chip for biochemical reactions, process control or analysis. The construction of this kind of chip is far more complex than microarray chips. According to ranges of its application, the chip can be further divided into three categories: sample pretreatment chip, reactive chip and analytical chip. They can perform complex and precise operations on microfluids (both liquid and gas), such as mixing and separation of microfluids, chemical reactions, microanalysis, etc. Besides, microfluidics chips also play a unique role in the screening of rare cells, the extraction and purification of MRNA, gene sequencing, single-cell analysis, and protein crystallization. They have been widely used in biotechnology research due to their lightweight, less sample or trial dose usage, fast reaction speed, large parallel processing and disposable features.
After the lecture, we conducted a questionnaire survey among participated students. From the feedback of more than 1,000 questionnaires collected, we can see that hundreds of students are full of interest in synthetic biology through this lecture, and students of different majors have made determination to take part in iGEM next year. (the questionnaire is followed)
