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Microbial Symphony
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By transforming these cells with our own plasmids, we can produce proteins of interest. This is the foundational goal of biomanufacturing.
Constitutive Expression
This system involves hookin a gene of interest up to a constitutive promoter, resulting in constant expression. The primary benefit of this "system" is its inherent simplicity - expression will always just occur. However, when optimizing yields, a flaw in this system becomes evident; low yields.
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1. Background
These are normal Escheri coli cells. Given nutrients and space, they will grow, producing various proteins.
Strain: Unmodified E. coli
Division Time: 60 Minutes
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Inducible Expression
This system involves hooking the gene in question up to an inducible promoter. Growth occurs an at an accelerated rate because no metabolic activity is put towards production. Once the culture has grown to an optimal density, an artificial inducer can be added, switching production on.
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