Team:Utrecht/Parts

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Several biobricks from the kit were used during our project. Some of these were isolated from the kit by direct transformation of 2 μl of DNA resuspended in elution buffer NE. Besides the biobricks that were isolated from the kit, some constructs were ordered completely from IDT (Table 1). These Biobricks were used to create several new biobricks that were relevant to our project. BBa_K2736000 and BBa_K2736001, were used as BRET pair to measure the activity of our customized Tar receptors BBa_K2736003, BBa_K2736004, and BBa_K2736005. To test whether it was possible to switch ligand binding domains of the Tar receptor and two component receptors we created BBa_K2736002, and BBa_K2736006.

Used Biobricks

Here comes Table 1

Created Biobricks

Here comes Table 2