Team:IIT Kanpur

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Sodium Dodecyl Sulphate (SDS) a component in shampoos, toothpastes and detergents accumulates in water bodies. It is toxic and foams, causing sewage problems

Our engineered E.coli DH5-α extracellularly releases alkyl sulfatase via attachment of N-terminal secretion signal sequence peIB

The action of alkyl sulfatase on SDS is that of degradation. The steps are stopped at 1-dodecanol

1-dodecanol is separated