Team:Aalto-Helsinki/Demonstrate

Demonstrate

Cloning

We assembled our constructs and cloned them into pSVA1 plasmid using Golden Gate assembly. After transforming plasmids to E.Coli Beta-10 and plasmid isolation we performed a check-up digestion with restriction enzymes NcoI and XhoI which cut the plasmid backbone close to BsaI insert site.

Protein expression and purification

We were able to successfully purify our silk containing construct prancerPurple-silk-CBM with the heat protocol

Cellulose binding

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Cellulose nanofibril binding

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Cotton binding

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Binding to hair keratin

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