For this year’s iGEM competition, our team has chosen two new parts to finish our project.
The first one is Nanobody with aldehyde-based precursor label, can be converted to fixed-point aldehyde-modified Nano (BBa_K2772000). It is anti-beta2 microglobulin nanobody with aldehyde-based precursor label, and it can be converted to fixed-point aldehyde-modified Nanobody by formylglycine-generating enzyme (FGE). It contains two sections: nanobody and ald tag, which is precursor of aldehyde label. Firstly it can cambein with beta2 microglobulin because of antigen-antibody interaction. Then its special lable allow fixed point fixing or other fixed point decoration possible.
Another one is Formylglycine-generating enzyme(BBa_K2772018). FGE can specifically identify ald-Tag, which is consisted of 6 base pair or 13 base pair, then it oxidze sulfhydryl group of sulfhydryl group to aldehyde group. Conveniently, it can be used for in vivo catalysis of the substrate label of the precursor, i.e. higher conversion can be achieved without purification separation.
Here is the list of our submitted parts.
| Name | Description | Length |
|---|---|---|
| BBa_K2772000 | Nanobody with aldehyde-based precursor label | 528bp |
| BBa_K2772018 | Formylglycine-generating enzyme | 863bp |
