Team:NDC-HighRiverAB/Attributions

The idea for our project, degrading fatbergs, was generated by one of our team members, Miguel, during one of our meetings. Our team then did research in the literature to determine what genes might be able to break down fats. Our mentors from FREDsense Technologies, Emily and Robert, helped us find relevant papers, identify our EstA gene and eventually design our genetic circuit, which we had synthesized by BioBasic.

Our project was made possible by help from a variety of different sources.

- Funding from geekStarter/Mindfuel, Knights of Columbus High River, Cargill High River, and Co-op High River. This allowed us to purchase our genetic circuit, purchase lab reagents and equipment as well as travel to places such as aGem 2018, and by the end of October, iGEM 2018.

- Throughout the process, our mentors, FREDsense’s Emily and Robert, helped us with the execution of different lab procedures as well as provided us advice which helped moved our project forward. Robert also provided the idea of testing our bacteria using Nitrophenol, which we were able to translate into figures, using a spectrophotometer with the help of the University of Calgary iGEM team.

- During aGEM, we were able to gain valuable responses from the judges and other participants, which helped us prepare for iGEM It was also during aGEM that we were able to contact Amino Labs and University of Calgary iGEM team who are helping us to mprove our presentation.

- A future project collaboration between our team and Amino Labs will also be discussed the meeting set on mid-October.

- George Dearing helped us with notarizing travel documents for iGEM, and Jayden Henderson helped us with photography.