Team:Queens Canada/gly

Glycerol Stocks · Benchling

Glycerol Stocks

Introduction

Make a bacterial glycerol stock for long term storage of the plasmid.

Materials

    • 50% sterilized glycerol (0.25uL pure glycerol, 0.25uL sterilized water)
    • *****Don does 900 uL of liquid culture and 100uL of pure 100% glycerol
    • liquid culture (prior overnight inoculated)

Procedure

  1. Label an eppendorf tube on side and top with: what plasmid, colony, QGEM.
  1. Combine 0.5uL of liquid culture in a sterilized 2mL eppendorf tube.
  1. Add 0.5mL of 50% glycerol.
  1. Vortex.
  1. Snap freeze in liquid nitrogen.
  1. Store at -80C