Team:TecCEM/Notebook
Notebook
13 - 19 May.
Summary
Research for characterization began and sub-projects deadlines were assigned. Logistics of the Latin American Meet-up began. A visit to Six Flags was made.
Wet lab
Preparation of solutions to be used for the competent cells. Transformation efficiency for E. coli DH5α and antibiotic viability were tested.
Dry lab
Delimitations of the mathematical model were made. Pitching of software ideas and sub-projects. We started looking into the possibility of creating a skinchip in a mini reactor. Investigation about the scaffold heterotrimer was appointed. The development of biobricks for recombinant leptin and collagen was started. A meeting with an expert in miRNA therapy was appointed, new insights about anti-miRNA were established from that meeting and the viability was researched. Leptin and collagen were codon optimized for their recombinant production in Escherichia coli BL21 (DE3) and Rosetta (DE3) respectively.
Human Practices
Committee for human practices was delimited. A brainstorming of ideas was done and the best ideas were picked up in order to develop them in the near future.
20 - 26 May.
Summary
Worklab began and we had meeting to assign new sub-project. Wiki page planification began, we contacted a Graphic designer to help out. We also contacted several specialists in tissue regeneration to gain insights about the project.
Wet lab
Lab got closed off for maintenance reasons. The iGEM kit got stuck at the customs border protection.
Dry lab
Skinchip 3D model was designed and software ideas were pitched. Biobricks were checked and revised. Chemical modifications for anti-miRNA were investigated, locked nucleic acid (LNA) technology appointed as the best method for miRNA silencing. Receptors within the scaffold for stem cell migration/call was investigated but also other molecules apart from the main project protein/molecules were investigated for the same purpose.
Human Practices
Fundraiser organization and planification, tasks were handed out. Went to a public talk about tissue regeneration in burn victims using nanoparticles of chitosan, we made contact with the people involved in the conference and received a feedback, another meeting with the experts was appointed. We had a meeting with a communication reporter to be published in a magazine. Online meet up with iGEM Brazil, Ecuador and Chile were performed.