Team:TUDelft/Acknowledgements

We have to thank many people that have helped us make our project become a reality. For the past nine months we received a lot of advice from various people, including our supervisors, stakeholders, sponsors and specialists.

While all of the practical work has been performed by the team the execution would not have been possible without all these people. This page is dedicated to everyone who helped us and believed in our project.

Next to everyone who helped we also have a special page for all our sponsors, without whom this project financially would not be possible.

Principal Investigators

• Prof. Stan Brouns
• Prof. Timon Idema

Supervisors

• Esengül Yildrim
• Cristóbal Almendros Romero
• Teunke van Rossum
• Ana Rita Costa
• Louis Reese
• David Foschepoth
• Franklin Luzia de Nóbrega
• Sebastian Kieper
• Zoë Robaey

General Support

• BN Secretaries
For helping with booking rooms for our endless meetings, sending packages and of course for giving us our iGEM coffee card.


• Jerwin de Graaf
From the communications department, for helping us with making our project movie and for his support in reaching out to the public.


• Lenny Bakker
For coaching us during the media training of TU Delft and for the feedback on important publications.


• Claire Hallewas
As our press officer of TU Delft, for the media support and sending the press release.


• Abigail Sison
For being our iGEM Headquarters contact person and helping us with all our iGEM related questions and deadlines.


• Michel van Baal
For giving us media training.


• Mark van Papelendam
For helping us staying on track with our finances.

Lab support

• Susanne Hage
For helping with the lab equipment and safety evaluations.


• Sasha Khaiboulov
For helping with the lab equipment and making our lab a great place to work at.


• James Brayer
For guiding us with the use of Oxford Nanopore Technologies.


• Theo van Laar - "The Library"
For always being willing to help and giving advice. Ranging in subjects from PCR optimisation, protein expression, purification, analysis and functionality. Het also provided us with a lot of consumables we required for the development of our fusion protein. Lastly he also gave us training in using chromatography equipment.


• Eli van der Sluis
For giving advice on protein expression, purification and analysis.


• Niels van der Broek
For performing mass spectroscopy on our Tn5 and dxCas9 proteins.


• Jan & Anke
For always providing us with autoclaved materials and also for bringing a lot of our packages.


• Michel Bengtson
For providing us with some extra Blood DNA extraction kits when we needed them the most.

Human Practices Support

• Hanna Stammes - Science Education and Communication TU Delft
For giving feedback on the high school lessons we organised.


• Jacco Gouw
For helping with editing of the general project movie.


• Remko Schoot Uiterkamp
For scheduling several lessons on a high school.


• Robin Sprinkhuizen
For scheduling several lessons on a high school.


• Cindy Stientstra & Anne Bosma
For scheduling several lessons on a high school where we could teach about synthetic biology and bioethics.


• Niek Savelkoul - RIVM
For giving feedback on the implementation of safety-by-design in our project.


• Korienke Smit - RIVM
For being our helpful contact person at the RIVM and giving useful advice on the safety-by-design.


• Cécile van der Vlugt - RIVM
For giving advice on the safety-by-design and pointing us towards possible unknown risks.


• Pim Klaassen, Jaco Westra, Pieter van Boheemen and Lilian van Hove - RIVM and the Rathenau Institute
For advising us on our safety by design.


• Olivier de Hon - Dutch Doping Authority
For providing us with information for the applied design during an interview.


• Daan Bregman - TU Delft Sports Engineering Institute
For connecting us to the TU Delft Sports Engineering Institute and its’ network.


• Dr. Paul Dimeo - Sterling University
For giving us advice on athlete behaviour and doping regulation, for bringing us in contact with many more experts and athletes and for hosting a seminar on gene doping and our project ADOPE.


• Jeroen van Bergen & Nahuel Paolini - ImmuneTune
For giving us an insight into the current status of gene therapy development.


• Arno Freeke, Luuk Goossen and Arend-Jan Krooneman - VR Institute TU Delft
For having interest in our VR plans and aiding in the realisation.


• Patrick van der Duin - Stichting Toekomstbeeld der Tehcniek
For looking into possibilities for a cooperation.


• Moniek Nijhuis - ex-Professional Swimster
For giving us her view on gene doping in an interview after having a close experience with a doping user.


• Dr. Colin Moran - Sterling University
For giving us his expert opinion on genetics and sports and for giving us a labtour around the facilities they use for sports and nutrition at Sterling University.


• Olivier Siegelaar - Professional Rower
For providing us with an insight on doping in rowing sports.


• James Ashworth
For providing us with the sight of an professional marathon runner.


• Everyone who filled in our survey and thereby gave us a good view on how (gene) doping fits in society

Drylab Support

• Dr. Joost Beltman - Leiden University
For validating our human body kinetics model.


• Hielke Walinga & Eduard Mucha
For helping and giving advice on the wiki.


• Jacob Hesse
For helping with constructing our collaborations wiki page.


• Misha Klein
For influencing our gRNA array model by helping us determine the number matching nucleotides required for the gRNA to bind.


• iGEM Aberdeen 2014
For building a great basis for the barcoding tool that we could use and improved to make a barcoding tool perfectly fitted to our project.


• Dr. Dorig H. Fürtinger
For providing advice in creating the EPO gene doping model.

Scientific Support

• Dr. Marieke von Lindern - Sanquin
For bringing us in contact with Sanquin.


• Aicha Ait Soussan - Sanquin
For teaching us on how to extract cell free DNA from blood plasma and serum.


• Dr. Ellen van der Schoot - Sanquin
For bringing us in contact with specialists in the field of cell free DNA.


• Onno Verhagen - Sanquin
For giving us a tour at Sanquin and introducing us on how Sanquin uses Next Generation Sequencing in their research.


• Dr. Chirlmin Joo & Viktorija Blobyte
For giving us advice on the properties of Cas-proteins and Transposases and for validating our proof of concept of our fusion protein at the start of our project.


• Prof. Hagan Bayley - Oxford University
For giving us advice on the enrichment of our samples and Oxford Nanopore Technologies.


• Rebecca McKenzie
For giving advice for the MinION sequencing module.


• Dr. Duncan McMillan
For advice in the early stages of the project and possible ways on how to dodge detection methods.


• Prof. Dr. Hidde Haisma - Groningen University & Gene and Cell Doping Expert Group WADA
For providing us with scientific information on gene doping and current detection methods.


• Dr. Mahadevan Lakshminarasimhan - Protein Biochemist at Modern Meadow
For giving advice on Cas9 purification and analysis.


• Dr. Lixin Dai - Director of Cell Engineering Modern Meadow
For giving advice on Tn5 purification and analysis and also for connecting us with other experts working on Tn5.


• Dr. William Reznikoff - Emeritus Professor University of Wisconsin-Madison
For giving advice on Tn5 functionality.


• Dr. Kim Remans - Head of Protein Expression and Purification Core Facility at the European Molecular Biology Laboratory
For giving advice and protocols on Tn5 purification, analysis and functionality testing.


• Dr. Anna Haagsma
For giving advice on Cas9 purification and analysis.


• Dr. Ivics Zoltán - Head of Medical Biotechnology Paul Ehrlich Institute
For giving us recommendations on which linker to use, advice on both Tn5 and Cas9 functionality and recommendation of other experts to reach out to.


• Dr. Lars M. Steinmetz - Senior Scientist and Director Life Science Alliance of the European Molecular Biology Laboratory
For sharing his research on Tn5 and for connecting us to Dr. Kim Remans and Dr. Bianca Hennig.


• Dr. Bianca Hennig - Postdoc at the European Molecular Biology Laboratory
For giving advice and protocols for the Tn5 purification, analysis and functionality testing.


• Dr. Alina Ham, Dr. Gerard Coyne & Dr. Angelica Vittori - Oxford Nanopore Technologies
For being our contact persons at Oxford Nanopore Technologies.


• Dr. Marie-Eve Aubin-Tam
For giving us advice on nanoparticle generation, safety for working with nanoparticles and the experiments.


• Dr. Robin Parisotto - Member UCI Athlete Biological Passport Panel
For giving us support and insight into the use of biological passports for doping detection.

EurAsian Meetup and Time in China

• Ms. Dorothy Zhang
For her invaluable contributions in organising the iGEM EurAsian Meetup, for giving a talk and workshop on “How to iGEM” during the meetup and for helping us arrange the other visits during our time in the People's Republic of China.


• The BGIC-Global iGEM Team 2018
For their help in organising the iGEM EurAsian Meetup and being a great team to mentor.


• Dr. Wei Li
For the valuable interview on isolating cell free DNA and sequencing methods and for sending us literature on these topics.


• Mr. Cao Jun
For the interesting conversation on the role of genetic information in sports.


• Dr. Doris Yang
For giving a short opening talk at the start of the iGEM EurAsian meetup.


• Dr. Yue (Chantal) Shen & Dr. Ying Gu
For giving an inspiring talk on genome-writing, eukaryotes and beyond during the iGEM EurAsian meetup.


• Ms. Hong Chen (Rebekah) - iGEM Ambassador
For giving an inspiring talk on after iGEM during the iGEM EurAsian meetup.


• Dr. Franklin Nobrega
For giving a great talk and workshop on How to iGEM during the iGEM EurAsian meetup.


• Ir. Britte Bouchaut
For giving an inspiring talk on bioethics during the EurAsian meetup and for joining us during the full meetup to give feedback and valuable insights to all teams there.


• Mr. Hui Kang
For giving an insightful talk on bioethics during the iGEM EurAsian meetup.


• Mr. Virgil Rerimassie
For his guidance in designing the bioethics workshop.


• SZU iGEM 2018 - Shenzhen University
For hosting us at their university to talk about iGEM and giving a great tour on their wonderful campus.


• Yantian Senior High School workers and their volunteers
For making this meet-up possible not only by providing us with a location but also with help throughout the meetup.

Fundraising Help and Advice

• Stefania Usai
For giving advice on our PCC grant application.


• Simon Klein
For giving general advice on fundraising.


• Jasper Veerman - iGEM TU Delft 2017
For giving general fundraising advice.


• Aizo Kroon
For giving a training in acquisition.


• Noa Leijdesdorff - iGEM Groningen 2018
For advice on running a crowdfunding.


• Jasqueline Leemkuil & Romy van Es
For helping us set up the crowdfunding page.


• Prof. Dr. Ir. Chris Kleijn
For valuable input on the application for the University Fund.

Random

• The Parents of Janine
For lending us their vacation home in Friesland for our teamweekend.


• Jeroen Jacques - iGEM TU Delft 2017
For taking our beautiful team pictures.


• Staff of the SHANGDONG HOTEL
For making sure we had a pleasant stay and providing us with their personal WiFi.


• Hajo Erwich and the Proteus ladies club eight rowing team
For letting us use their rowing film material.


• De Efteling
For being the happiest place in The Netherlands to escape to with the team when the Wiki Freeze is approaching.

Crowdfunding

We want to thank everyone who donated to our crowdfunding. Without this money we wouldn’t have been able to complete our project and this gave us the last necessary push!




• Claudia
• Beivid
• Marcel
• Ronald
• Wim
• Tatjana
• Anonymous et al.