Crosslinking
By taking advantage of the covalent crosslinking of SpyTag and SpyCatcher, a polymer network was designed to possess special properties, such as strong mechanical strength, large contact area and stable structure.
Secretion
To make the polymer network reproductive and economical, a signal peptide library was constructed. We carried out a series of experiments to find an optimal secretion strategy.
Clearance
The monomeric streptavidin constructed in the polymer network could realize the clearance of Spy Crosslinking Network in the environment by interacting with biotinylated magnetic beads.
Model
To accurately describe the Gel Point (GP) and the Mass Distribution (MD) for our polymer network, we built up two amendatory models that can adequately describe the characters and deepen our knowledge of our novel biomaterial.
Software
To calculate the Gel Point (GP) and the Mass Distribution (MD) for our polymer network, we established a useful software called SoP that can apply to all the crosslinking reactions between the chosen monomers.
Human practices
The Peking iGEM 2016 focuses on current treatments of uranium pollution worldwide, and looks for solutions using synthetic biology. We visited a institution of nuclear industry, conducted a questionnaire survey and provided public education.