Team:WHU-China/Notebook/Lab2

IGEM-WHU

Notebook of algae-bacteria symbiotic laboratory

18.05.11
Determine the medium, the growth curve of the algae - to determine the species of the algae, set the alternative species, the initial culture conditions are determined, the focus is on the filamentous green algae (can not be quantified, different traits in different periods, the cultivation is more difficult , want to use non-synthetic biological methods to improve the stability of the film light stress)
18.05.13
got a variety of algae species
The indicator of growth status is determined 1: film thickness (excluded later)
Culture conditions: culture fluid circulation
Digital model: find the most significant amount of the growth rate of algae as the main variable of the post-experiment, the input and output have been formed
Growth indicator 2: dry weight, reactive dyes, enzyme activity
The preliminary plan for the preliminary experiment of 0518 Department of Biosynthesis:
18.05.19
E. coli vs. Pseudomonas putida, E. coli wins
18.05.24
bacteria algae than experimental protocol
18.05.26
Centrifugal separation of algae, let the algae completely separate od Indicator 3 of growth status: chlorophyll
18.06.08
bacteria algae than large experimental data
18.07.04
filtration method, sub-bacterial algae test, failure
18.07.19
proposed two lines - suspension system + preliminary conclusion of suspension system (medium, bacteria and algae ratio) small plastic cup experiment - attempted film extraction
Suspension culture experiments monitored dynamic growth indicators (modeling) used: blood count and chlorophyll
Small experiment of culture dish: improved experiment of film extraction Pre-experiment of blood cell count: standard od and number
18.07.24
The experiment encountered a bottleneck
The literature supports peak bacteria and algae ratio 3:1
Try the signal molecule of the conditional algae exchange - self-sensit
18.07.26
Try a variety of membrane materials and membrane materials than the initial test
18.07.28
Method for establishing membrane shedding rate - shaker, peristaltic pump
18.07.29
screening membrane material combination
18.07.30
making mixer device
18.08.03
mixing device 6 completed
Improvement of growth status indicators: chlorophyll
18.09.08
Growth status indicator: CFU method for measuring algae Culture system BG11 improved-BG11
18.09.13
C. algae CFU experiment began to do
18.09.22
Summary: Comparison of the various methods of counting
18.09.23
Dual Wavelength Spectrophotometry
Algae gradient full-band scanning
18.10.07
Using existing standard systems to verify the effect of parts on membrane stability



Part of our Raw data

I.Suspension co-culture conditions

1. culture medium of co-culture

alage:

0.232	0.321	0.386	0.445	0.411	0.405	0.4
0.264	0.376	0.419	0.434	0.441	0.413	0.421
0.201	0.269	0.34	0.342	0.336	0.282	0.3
0.129	0.103	0.082	0.071	0.079	0.069	0.078
0.095	0.063	0.067	0.078	0.04	0.082	0.063
0.132	0.173	0.116	0.155	0.142	0.168	0.158
0.143	0.141	0.112	0.114	0.097	0.099	0.097
0.118	0.11	0.114	0.118	0.056	0.071	0.061

0.253	0.342	0.407	0.466	0.432	0.426	0.421
0.285	0.397	0.44	0.455	0.462	0.434	0.442
0.222	0.29	0.361	0.363	0.357	0.303	0.321
0.15	0.124	0.103	0.092	0.1	0.09	0.099
0.116	0.084	0.088	0.099	0.061	0.103	0.084
0.156	0.197	0.14	0.179	0.166	0.192	0.182
0.167	0.165	0.136	0.138	0.121	0.123	0.121
0.142	0.134	0.138	0.142	0.08	0.095	0.085
	1	2	3	4	5	6	7
9:1	1.265	1.71	2.035	2.33	2.16	2.13	2.105
7:1	1.425	1.985	2.2	2.275	2.31	2.17	2.21
4:1	1.11	1.45	1.805	1.815	1.785	1.515	1.605
2:1	0.75	0.62	0.515	0.46	0.5	0.45	0.495
1:2	0.58	0.42	0.44	0.495	0.305	0.515	0.42
1:4	0.39	0.4925	0.35	0.4475	0.415	0.48	0.455
1:7	0.4175	0.4125	0.34	0.345	0.3025	0.3075	0.3025
1:9	0.355	0.335	0.345	0.355	0.2	0.2375	0.2125

bacteria

0.311	0.419	0.538	0.567	0.511	0.422	0.361
0.357	0.484	0.577	0.538	0.53	0.483	0.421
0.27	0.34	0.414	0.39	0.371	0.284	0.3
0.164	0.104	0.063	0.061	0.003	0.011	0.004
0.113	0.082	0.061	0.017	0.022	0.034	0.024
0.169	0.2	0.1	0.128	0.083	0.099	0.087
0.182	0.159	0.092	0.078	0.032	0.031	0.034
0.149	0.124	0.094	0.097	-0.012	0.003	0.01

0.384	0.492	0.611	0.64	0.584	0.495	0.434
0.43	0.557	0.65	0.611	0.603	0.556	0.494
0.343	0.413	0.487	0.463	0.444	0.357	0.373
0.237	0.177	0.136	0.134	0.076	0.084	0.077
0.186	0.155	0.134	0.09	0.095	0.107	0.097
0.244	0.275	0.175	0.203	0.158	0.174	0.162
0.257	0.234	0.167	0.153	0.107	0.106	0.109
0.224	0.199	0.169	0.172	0.063	0.078	0.085
	1	2	3	4	5	6	7
9:1	1.92	2.46	3.055	3.2	2.92	2.475	2.17
7:1	2.15	2.785	3.25	3.055	3.015	2.78	2.47
4:1	1.715	2.065	2.435	2.315	2.22	1.785	1.865
2:1	1.185	0.885	0.68	0.67	0.38	0.42	0.385
1:2	0.93	0.775	0.67	0.45	0.475	0.535	0.485
1:4	0.61	0.6875	0.4375	0.5075	0.395	0.435	0.405
1:7	0.6425	0.585	0.4175	0.3825	0.2675	0.265	0.2725
1:9	0.56	0.4975	0.4225	0.43	0.1575	0.195	0.2125

2. Determine the optimal ratio of bacteria to algae

Initial value	OD:  bacteria：algae	Three parallel Per groups
group	①	②	③	④
proportion	0:1	1:100	1:10	1:1
Actual absorbance	00:00.2	0.002：0.2	0.02 : 0.2	0.2 : 0.2

time/h	group 1	group 2	group 3	group 4
17.5	0	0.006	0.162	0.426
41	0.03	0.018	0.174	0.444
67	0.036	0.108	0.204	0.336
86	0.15	0.216	0.3	0.612
114.5	0.126	0.186	0.42	0.624
160	0.096	0.138	0.21	0.432
186.5	0.174	0.156	0.204	0.348
210.5	0.264	0.222	0.216	0.258
234.5	0.216	0.18	0.258	0.216

time/h	1;1	1;2	1;3	2;1	2;2	2;3	3;1	3;2	3;3	4;1	4;2	4;3
17.5	-0.03	-0.012	0.054	0.006	0.018	-0.006	0.132	0.222	0.132	0.456	0.408	0.42
41	0.012	0.03	0.054	0.012	0.024	0.03	0.186	0.192	0.156	0.462	0.36	0.51
67	0.012	0.018	0.084	0.072	0.126	0.126	0.132	0.15	0.15	0.312	0.318	0.39
86	0.192	0.132	0.126	0.252	0.216	0.186	0.252	0.306	0.342	0.6	0.678	0.558
114.5	0.126	0.12	0.138	0.15	0.204	0.216	0.432	0.42	0.408	0.684	0.564	0.63
160	0.138	0.126	0.024	0.126	0.12	0.168	0.198	0.204	0.234	0.42	0.438	0.444
186.5	0.198	0.192	0.144	0.198	0.144	0.132	0.18	0.228	-0.024	0.24	0.456	0.354
210.5	0.264	0.258	0.276	0.198	0.27	0.198	0.162	0.264	-0.024	0.258	0.24	0.276
234.5	0.204	0.228	0.222	0.144	0.228	0.168	0.246	0.27	-0.012	0.294	0.222	0.138

time/h	group 1	group 2	group 3	group 4
17.5	0.506	0.332	0.56	0.674
41	0.762	0.766	0.87	1.124
67	1.138	1.112	1.216	1.476
86	1.13	1.094	1.262	1.474
114.5	1.264	1.282	1.404	1.662
160	1.18	1.232	1.406	1.766
186.5	1.368667	1.401333	1.646	2.114
210.5	1.272	1.298667	1.588	1.890667
234.5	1.26	1.267333	1.56	2.126667

time/h	1;1	2;1	3;1	4;1	1;2	2;2	3;2	4;2	1;3	2;3	3;3	4;3
17.5	0.241	0.129	0.247	0.377	0.243	0.202	0.317	0.374	0.276	0.168	0.276	0.261
41	0.383	0.389	0.439	0.574	0.365	0.372	0.434	0.566	0.395	0.389	0.433	0.547
67	0.579	0.525	0.649	0.766	0.572	0.549	0.61	0.71	0.558	0.596	0.565	0.74
86	0.56	0.526	0.652	0.749	0.579	0.526	0.643	0.711	0.556	0.59	0.6	0.753
114.5	0.6	0.611	0.738	0.836	0.656	0.642	0.7	0.806	0.642	0.67	0.668	0.851
160	0.643	0.64	0.756	0.91	0.548	0.652	0.722	0.901	0.581	0.556	0.631	0.839
186.5	0.784	0.665	0.875	1.184	0.649	0.676	0.771	0.999	0.62	0.761	0.393	0.988
210.5	0.699	0.636	0.855	0.969	0.599	0.656	0.733	0.932	0.61	0.656	0.4	0.935
234.5	0.682	0.612	0.83	0.993	0.525	0.643	0.73	0.953	0.683	0.646	0.426	1.244

II. Biofilm formation and culture conditions established

Number	materials	Diameter of pores/μm	ration	stability
1	Mixed fiber membrane + blotting paper	0.45	10:1(3.5x10^7)	×
2	Mixed film + toilet paper	0.45	10:1	×
3	Polypropylene film + absorbent paper	0.1	10:1	×
4	Absorbent paper	/	10:1	×
5	Writing paper	/	10:1	×
6	Mixed film + writing paper	0.45	10:1	×
7	Polypropylene film + writing paper	0.1	10:1	×
8	Mixed fiber membrane	0.45	10:1	×
9	Mixed fiber membrane	0.22	10:1	×
10	Mixed fiber membrane	3	10:1	×
11	Mixed fiber membrane	0.45	20:2	×
12	Mixed fiber membrane	3	20:2	×
13	Mixed fiber membrane	3	10:2	×
14	Mixed fiber membrane	0.22	5:1	√
15	Mixed fiber membrane	0.45	5:1	√
16	Mixed fiber membrane	0.22	3:1	√
17	Mixed fiber membrane	0.45	3:1	√
18	Mixed film (front)	3	3:1	×
19	Qualitative filter paper	/	10:1	√
20	Qualitative filter paper	/	20:2	√
21	Qualitative filter paper	/	5:1	√
22	Qualitative filter paper	/	3:1	√
23	cotton	/	10:1	√
24	Mixed film (reverse)	3	3:1	×
25	Cloth	/	10:1	√
26	Cloth	/	5:1	√
27	Cloth	/	20;2	√
28	Cloth	/	3:1	√
29	Mixed fiber membrane	3	10:1	×
30	Mixed fiber membrane	3	5:1	×
31	Mixed fiber membrane	3	3:1	×
32	Mixed fiber membrane	3	20:2	√
33	Mixed fiber membrane	3	20:1	×
34	Mixed fiber membrane	3	50:1	√
35	Mixed fiber membrane	3	100:1	×
36	Mixed fiber membrane	0.45	10:1	√
37	Mixed fiber membrane	0.45	20:1	×
38	Mixed fiber membrane	0.45	50:1	×
39	Mixed fiber membrane	0.45	100:1	×
40	Mixed fiber membrane	0.45	5:1	×
41	Mixed fiber membrane	0.22	10:1	×
42	Mixed fiber membrane	0.22	20:1	×
43	Mixed fiber membrane	0.22	5:1	×
44	Mixed fiber membrane	0.22	3:1	×
45	Mixed fiber membrane	3	50:1	×
46	Mixed fiber membrane	0.22	10:2	×
47	Mixed film (front)	3	10:4	√
48	Mixed film (reverse)	3	10:4	√

III. Establishment of standard method to evaluate the stability of the biofilm

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
1-1A	1.23	0.793	1.622	1.508	1.08	1.922	20.99186	1-1	0.270319
1-1B	0.233	0.2	0.423	0.511	0.487	0.723	7.77669
1-2A	0.867	0.55	1.151	1.145	0.837	1.451	15.81377	1-2	0.314562
1-2B	0.272	0.189	0.378	0.55	0.476	0.678	7.25726
1-3A	0.72	0.46	0.987	0.998	0.747	1.287	14.02073	1-3	0.466449
1-3B	0.609	0.395	0.829	0.887	0.682	1.129	12.25741
1-4A	0.659	0.437	0.921	0.937	0.724	1.221	13.27893	1-4	0.57297
1-4B	1.073	0.662	1.336	1.351	0.949	1.636	17.81706
1-5A	0.813	0.574	1.2	1.091	0.861	1.5	16.36178	1-5	0.660063
1-5B	0.846	0.551	1.157	2.248	1.676	2.914	31.77002
1-6A	0.955	0.645	1.377	1.233	0.932	1.677	18.33853	1-6	0.547671
1-6B	0.546	0.356	0.726	1.648	1.286	2.052	22.20392

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
1-1A	0.398	0.362	0.756	0.676	0.649	1.056	11.46006	1-1	0.624916
1-1B	0.92	0.68	1.445	1.198	0.967	1.745	19.09323
1-2A	0.532	0.39	0.858	0.81	0.677	1.158	12.61492	1-2	0.538813
1-2B	0.71	0.464	1.048	0.988	0.751	1.348	14.73822
1-3A	0.255	0.232	0.492	0.533	0.519	0.792	8.5433	1-3	0.669452
1-3B	0.919	0.578	1.276	0.512	0.865	1.576	17.30254
1-4A	0.234	0.177	0.38	1.141	0.464	0.68	7.25216	1-4	0.698348
1-4B	0.863	0.549	1.229	0.524	0.836	1.529	16.78929
1-5A	0.246	0.206	0.434	0.984	0.493	0.734	7.85996	1-5	0.643324
1-5B	0.706	0.445	0.995	0.522	0.732	1.295	14.17671
1-6A	0.244	0.228	0.458	1.045	0.515	0.758	8.1056	1-6	0.656481
1-6B	0.767	0.482	1.109	0.278	0.769	1.409	15.49015

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
2-1A	1.232	0.843	1.703	1.51	1.13	2.003	21.87455	2-1	0.207334
2-1B	0.168	0.122	0.239	0.446	0.409	0.539	5.72161
2-2A	1.176	0.811	1.604	1.454	1.098	1.904	20.75516	2-2	0.309094
2-2B	0.405	0.281	0.562	0.683	0.568	0.862	9.28534
2-3A	0.903	0.657	1.32	1.181	0.944	1.62	17.64876	2-3	0.428306
2-3B	0.66	0.443	0.917	0.938	0.73	1.217	13.22221
2-4A	0.905	0.62	1.228	1.183	0.907	1.528	16.61538	2-4	0.441088
2-4B	0.665	0.46	0.91	0.943	0.747	1.21	13.11268
2-5A	0.987	0.656	1.328	1.265	0.943	1.628	17.73838	2-5	0.381236
2-5B	0.478	0.341	0.709	0.756	0.628	1.009	10.92905
2-6A	1.308	0.874	1.697	1.586	1.161	1.997	21.74963	2-6	0.36848
2-6B	0.679	0.441	0.872	0.957	0.728	1.172	12.69052

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
2-1A	0.564	0.429	0.877	0.842	0.716	1.177	12.77745	2-1	0.596511
2-1B	0.981	0.678	1.428	1.259	0.965	1.728	18.88998
2-2A	0.61	0.505	1.062	0.888	0.792	1.362	14.84898	2-2	0.52318
2-2B	0.89	0.561	1.193	1.168	0.848	1.493	16.29269
2-3A	0.414	0.3	0.635	0.692	0.587	0.935	10.12041	2-3	0.645336
2-3B	1	0.647	1.384	1.278	0.934	1.684	18.4148
2-4A	0.305	0.222	0.47	0.583	0.509	0.77	8.294	2-4	0.710082
2-4B	1.152	0.737	1.557	1.43	1.024	1.857	20.31409
2-5A	0.524	0.451	0.93	0.802	0.738	1.23	13.37482	2-5	0.627275
2-5B	1.34	0.864	1.76	1.618	1.151	2.06	22.50902
2-6A	0.429	0.38	0.77	0.707	0.667	1.07	11.59576	2-6	0.639574
2-6B	1.517	0.763	1.585	1.795	1.05	1.885	20.57665

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
3-1A	1.786	1.18	2.119	2.064	1.467	2.419	26.24085	3-1	0.205789
3-1B	6.275	0.213	0.383	6.553	0.5	0.683	6.79931
3-2A	1.521	0.967	1.894	1.799	1.254	2.194	23.92382	3-2	0.210971
3-2B	0.212	0.143	0.299	0.49	0.43	0.599	6.39675
3-3A	1.482	0.95	1.872	1.76	1.237	2.172	23.69242	3-3	0.240367
3-3B	0.291	0.194	0.399	0.569	0.481	0.699	7.49689
3-4A	1.632	1.049	1.982	1.91	1.336	2.282	24.83146	3-4	0.259145
3-4B	0.374	0.241	0.506	0.652	0.528	0.806	8.68582
3-5A	1.578	1.011	1.979	1.856	1.298	2.279	24.85875	3-5	0.394575
3-5B	0.666	0.532	1.18	0.944	0.819	1.48	16.20122
3-6A	1.145	0.897	1.71	1.423	1.184	2.01	21.8813	3-6	0.324573
3-6B	0.495	0.332	0.673	0.773	0.619	0.973	10.51495

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
3-1A	0.885	0.548	1.109	1.163	0.835	1.409	15.31771	3-1	0.394527
3-1B	0.496	0.317	0.626	0.774	0.604	0.926	9.98102
3-2A	0.82	0.51	1.036	1.098	0.797	1.336	14.51638	3-2	0.418606
3-2B	0.503	0.311	0.665	0.781	0.598	0.965	10.45185
3-3A	0.882	0.548	1.146	1.16	0.835	1.446	15.7564	3-3	0.333124
3-3B	0.313	0.204	0.432	0.591	0.491	0.732	7.87078
3-4A	0.824	0.513	1.094	1.102	0.8	1.394	15.19874	3-4	0.456218
3-4B	0.631	0.404	0.872	0.909	0.691	1.172	12.75134
3-5A	0.827	0.533	1.101	1.105	0.82	1.401	15.25065	3-5	0.445687
3-5B	0.601	0.377	0.827	0.879	0.664	1.127	12.26207
3-6A	0.774	0.482	1.038	1.052	0.769	1.338	14.58688	3-6	0.405307
3-6B	0.432	0.269	0.616	0.71	0.556	0.916	9.94156

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
1-1A	1.838	1.334	1.878	2.116	1.621	2.178	23.14368	1-1	0.152838
1-1B	-0.002	-0.104	0.078	0.276	0.183	0.378	4.1754
1-2A	1.959	1.787	1.897	2.237	2.074	2.197	22.66153	1-2	0.15882
1-2B	0.002	-0.102	0.087	0.28	0.185	0.387	4.27865
1-3A	1.96	1.833	1.885	2.238	2.12	2.185	22.44841	1-3	0.182859
1-3B	0.061	-0.063	0.168	2.217	0.224	0.468	5.02348
1-4A	1.939	1.676	1.883	0.225	1.963	2.183	22.82753	1-4	0.130565
1-4B	-0.053	-0.128	0.025	2.229	0.159	0.325	3.42807
1-5A	1.951	1.879	1.831	0.425	2.166	2.131	21.88271	1-5	0.223914
1-5B	0.147	0	0.282	1.765	0.287	0.582	6.31352
1-6A	1.487	0.904	1.686	0.167	1.191	1.986	21.6866	1-6	0.104997
1-6B	-0.111	-0.176	-0.069	0.278	0.111	0.231	2.54417

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
2-1A	1.476	1.2	1.853	1.754	1.487	2.153	23.08275	2-1	0.262895
2-1B	0.256	0.072	0.445	0.534	0.359	0.745	8.23267
2-2A	1.785	1.276	1.831	2.063	1.563	2.131	22.68029	2-2	0.472915
2-2B	1.427	0.846	1.576	1.705	1.133	1.876	20.34938
2-3A				0.278	0.287	0.3	3.09078	2-3
2-3B				0.278	0.287	0.3	3.09078
2-4A	1.87	1.501	1.812	2.148	1.788	2.112	22.10184	2-4	0.352981
2-4B	0.559	0.258	0.794	0.837	0.545	1.094	12.05764
2-5A	1.856	1.524	1.765	2.134	1.811	2.065	21.51059	2-5	0.451895
2-5B	1.058	0.57	1.317	1.336	0.857	1.617	17.73479
2-6A	1.707	1.136	1.78	1.985	1.423	2.08	22.29778	2-6	0.390238
2-6B	0.778	0.395	1	1.056	0.682	1.3	14.27024

				Correction value
group number	λ666	λ653	λ470	λ666	λ653	λ470	c		Shedding rate
3-1A	1.832	1.315	1.872	2.11	1.602	2.172	23.10232	3-1	0.256887
3-1B	0.23	0.041	0.42	0.508	0.328	0.72	7.98624
3-2A	1.891	1.496	1.84	2.169	1.783	2.14	22.43966	3-2	0.283524
3-2B	0.294	0.073	0.5	0.572	0.36	0.8	8.87984
3-3A	1.845	1.372	1.832	2.123	1.659	2.132	22.5395	3-3	0.340541
3-3B	0.484	0.195	0.75	0.762	0.482	1.05	11.63926
3-4A	1.734	1.155	1.837	2.012	1.442	2.137	22.94181	3-4	0.340739
3-4B	0.507	0.206	0.77	0.785	0.493	1.07	11.85748
3-5A	1.577	0.993	1.726	1.855	1.28	2.026	21.8885	3-5	0.386312
3-5B	0.63	0.291	0.944	0.908	0.578	1.244	13.77864
3-6A	1.823	1.288	1.87	2.101	1.575	2.17	23.12092	3-6	0.263178
3-6B	0.249	0.048	0.444	0.527	0.335	0.744	8.25834

Name	Count	Shedding rate		Name			Count	Shedding rate		Name			Count	Shedding rate
1-1-A1.b	587	0.324511		1	1	A1.bmp	363	0.305927								Name				Count
1-1-A2.b	486	0.370466		2	1	A1.bmp	493	0.295714								1	1	A1.bmp	0.65362	177
1-2-A1.b	107	0.548523		3	1	A1.bmp	270	0.429175		1	1	A1.bmp	0.401389	431		1	1	A2.bmp	0.558577	211
1-2-A2.b	191	0.39557		1	1	A2.bmp	361	0.188764		2	1	A1.bmp	0.566441	385		2	1	A1.bmp	0.65917	197
1-3-A1.b	445	0.589104		2	1	A2.bmp	376	0.304991		3	1	A1.bmp	0.437247	278		2	1	A2.bmp	0.590361	204
1-3-A2.b	536	0.557391		3	1	A2.bmp	310	0.376258		1	1	A2.bmp	0.43909	419		3	1	A1.bmp	0.472934	185
1-4-A1.b	264	0.649867		1	1	B1.bmp	160			2	1	A2.bmp	0.61215	332		3	1	A2.bmp	0.467085	170
1-4-A2.b	203	0.713681		2	1	B1.bmp	207			3	1	A2.bmp	0.389277	262		1	2	A1.bmp	0.632495	190
1-5-A1.b	224	0.68		3	1	B1.bmp	203			1	2	A1.bmp	0.514739	214		1	2	A2.bmp	0.618304	171
1-5-A2.b	206	0.725699		1	1	B2.bmp	84			2	2	A1.bmp	0.633867	320		2	2	A1.bmp	0.613095	195
1-6-A1.b	260	0.651475		2	1	B2.bmp	165			3	2	A1.bmp	0.697616	241		2	2	A2.bmp	0.583541	167
1-6-A2.b	305	0.621588		3	1	B2.bmp	187			1	2	A2.bmp	0.570796	194		3	2	A1.bmp	0.763209	121
2-1-A1.b	413	0.258528		1	2	A1.bmp	392	0.395062		2	2	A2.bmp	0.652387	284		3	2	A2.bmp	0.670082	161
2-1-A2.b	353	0.206742		2	2	A1.bmp	377	0.486376		3	2	A2.bmp	0.65	287		1	3	A1.bmp	0.649798	173
2-2-A1.b	409	0.30678		3	2	A1.bmp	330	0.495413		1	3	A1.bmp	0.469914	370		1	3	A2.bmp	0.571956	232
2-2-A2.b	440	0.376771		1	2	A2.bmp	339	0.447883		2	3	A1.bmp	0.520286	201		2	3	A1.bmp	0.717626	157
2-3-A1.b	905	0.349856		2	2	A2.bmp	247	0.606688		3	3	A1.bmp	0.541374	327		2	3	A2.bmp	0.736287	125
2-3-A2.b	974	0.284875		3	2	A2.bmp	325	0.497682		1	3	A2.bmp	0.428148	386		3	3	A1.bmp	0.518519	182
2-4-A1.b	293	0.691254		1	2	B1.bmp	256			2	3	A2.bmp	0.485294	210		3	3	A2.bmp	0.693252	150
2-4-A2.b	231	0.724344		2	2	B1.bmp	357			3	3	A2.bmp	0.542484	350		1	4	A1.bmp	0.517007	284
2-5-A1.b	321	0.682178		3	2	B1.bmp	324			1	4	A1.bmp	0.497436	294		1	4	A2.bmp	0.473934	222
2-5-A2.b	247	0.723094		1	2	B2.bmp	275			2	4	A1.bmp	0.387608	425		2	4	A1.bmp	0.679389	168
2-6-A1.b	332	0.736926		2	2	B2.bmp	381			3	4	A1.bmp	0.614545	318		2	4	A2.bmp	0.686441	148
2-6-A2.b	320	0.772566		3	2	B2.bmp	322			1	4	A2.bmp	0.516611	291		3	4	A1.bmp	0.465328	293
3-1-A1.b	687	0.375455		1	3	A1.bmp	290	0.451796		2	4	A2.bmp	0.446959	391		3	4	A2.bmp	0.559415	241
3-1-A2.b	713	0.360538		2	3	A1.bmp	300	0.563319		3	4	A2.bmp	0.595062	328		1	5	A1.bmp	0.723539	175
3-2-A1.b	435	0.358407		3	3	A1.bmp	158	0.649667		1	5	A1.bmp	0.396936	433		1	5	A2.bmp	0.552083	215
3-2-A2.b	453	0.329882		1	3	A2.bmp	302	0.419231		2	5	A1.bmp	0.408618	398		2	5	A1.bmp	0.65897	192
3-3-A1.b	396	0.644843		2	3	A2.bmp	206	0.615672		3	5	A1.bmp	0.501269	393		2	5	A2.bmp	0.686992	154
3-3-A2.b	415	0.632743		3	3	A2.bmp	182	0.712934		1	5	A2.bmp	0.418429	385		3	5	A1.bmp	0.714286	164
3-4-A1.b	439	0.624786		1	3	B1.bmp	239			2	5	A2.bmp	0.485755	361		3	5	A2.bmp	0.697588	163
3-4-A2.b	348	0.657817		2	3	B1.bmp	387			3	5	A2.bmp	0.529412	336		1	6	A1.bmp	0.434091	249
3-5-A1.b	325	0.773519		3	3	B1.bmp	293			1	6	A1.bmp	0.570565	213		1	6	A2.bmp	0.521411	190
3-5-A2.b	336	0.753485		1	3	B2.bmp	218			2	6	A1.bmp	0.536716	347		2	6	A1.bmp	0.565121	197
3-6-A1.b	319	0.708676		2	3	B2.bmp	330			3	6	A1.bmp	0.520301	319		2	6	A2.bmp	0.452174	252
3-6-A2.b	364	0.71316		3	3	B2.bmp	452			1	6	A2.bmp	0.547655	299		3	6	A1.bmp	0.64775	180
				1	4	A1.bmp	144	0.518395		2	6	A2.bmp	0.553398	276		3	6	A2.bmp	0.734317	144
1-1-B1.b	282			2	4	A1.bmp	320	0.580603		3	6	A2.bmp	0.471891	310
1-1-B2.b	286			3	4	A1.bmp	163	0.757079		1	1	B1.bmp		289
1-2-B1.b	130			1	4	A2.bmp	149	0.404		1	2	B1.bmp		227		4	1	A1.bmp	0.643369	199
1-2-B2.b	125			2	4	A2.bmp	216	0.594747		1	3	B1.bmp		328		4	1	A2.bmp	0.509158	268
1-3-B1.b	638			3	4	A2.bmp	158	0.692607		1	4	B1.bmp		291		5	1	A1.bmp	0.696774	235
1-3-B2.b	675			1	4	B1.bmp	155			1	5	B1.bmp		285		5	1	A2.bmp	0.604588	293
1-4-B1.b	490			2	4	B1.bmp	443			1	6	B1.bmp		283		6	1	A1.bmp	0.641068	215
1-4-B2.b	506			3	4	B1.bmp	508			2	1	B1.bmp		503		6	1	A2.bmp	0.638264	225
1-5-B1.b	476			1	4	B2.bmp	101			2	2	B1.bmp		554		4	2	A1.bmp	0.749169	151
1-5-B2.b	545			2	4	B2.bmp	317			2	3	B1.bmp		218		4	2	A2.bmp	0.849421	78
1-6-B1.b	486			3	4	B2.bmp	356			2	4	B1.bmp		269		5	2	A1.bmp	0.741214	162
1-6-B2.b	501			1	5	A1.bmp	270	0.300518		2	5	B1.bmp		275		5	2	A2.bmp	0.751984	125
2-1-B1.b	144			2	5	A1.bmp	218	0.581574		2	6	B1.bmp		402		6	2	A1.bmp	0.772616	186
2-1-B2.b	92			3	5	A1.bmp	208	0.665056		3	1	B1.bmp		216		6	2	A2.bmp	0.801757	158
2-2-B1.b	181			1	5	A2.bmp	216	0.345455		3	2	B1.bmp		556		4	3	A1.bmp	0.692057	283
2-2-B2.b	266			2	5	A2.bmp	170	0.719472		3	3	B1.bmp		386		4	3	A2.bmp	0.639286	303
2-3-B1.b	487			3	5	A2.bmp	236	0.641337		3	4	B1.bmp		507		5	3	A1.bmp	0.809079	143
2-3-B2.b	388			1	5	B1.bmp	116			3	5	B1.bmp		395		5	3	A2.bmp	0.814768	148
2-4-B1.b	656			2	5	B1.bmp	303			3	6	B1.bmp		346		6	3	A1.bmp	0.663717	190
2-4-B2.b	607			3	5	B1.bmp	413			1	1	B2.bmp		328		6	3	A2.bmp	0.748239	143
2-5-B1.b	689			1	5	B2.bmp	114			1	2	B2.bmp		258		4	4	A1.bmp	0.744624	190
2-5-B2.b	645			2	5	B2.bmp	436			1	3	B2.bmp		289		4	4	A2.bmp	0.70936	177
2-6-B1.b	930			3	5	B2.bmp	422			1	4	B2.bmp		311		5	4	A1.bmp	0.72103	195
2-6-B2.b	1087			1	6	A1.bmp	129	0.724359		1	5	B2.bmp		277		5	4	A2.bmp	0.814229	141
3-1-B1.b	413			2	6	A1.bmp	157	0.761035		1	6	B2.bmp		362		6	4	A1.bmp	0.808889	129
3-1-B2.b	402			3	6	A1.bmp	101	0.827055		2	1	B2.bmp		524		6	4	A2.bmp	0.8448	97
3-2-B1.b	243			1	6	A2.bmp	125	0.734607		2	2	B2.bmp		533		4	5	A1.bmp	0.755072	169
3-2-B2.b	223			2	6	A2.bmp	137	0.777597		2	3	B2.bmp		198		4	5	A2.bmp	0.809295	119
3-3-B1.b	719			3	6	A2.bmp	73	0.870567		2	4	B2.bmp		316		5	5	A1.bmp	0.864516	105
3-3-B2.b	715			1	6	B1.bmp	339			2	5	B2.bmp		341		5	5	A2.bmp	0.809976	160
3-4-B1.b	731			2	6	B1.bmp	500			2	6	B2.bmp		342		6	5	A1.bmp	0.70232	231
3-4-B2.b	669			3	6	B1.bmp	483			3	1	B2.bmp		167		6	5	A2.bmp	0.681885	216
3-5-B1.b	1110			1	6	B2.bmp	346			3	2	B2.bmp		533		4	6	A1.bmp	0.693452	206
3-5-B2.b	1027			2	6	B2.bmp	479			3	3	B2.bmp		415		4	6	A2.bmp	0.753205	154
3-6-B1.b	776			3	6	B2.bmp	491			3	4	B2.bmp		482		5	6	A1.bmp	0.736342	222
3-6-B2.b	905									3	5	B2.bmp		378		5	6	A2.bmp	0.753482	177
										3	6	B2.bmp		277		6	6	A1.bmp	0.720772	246
4-1-A1.b	380	0.37397		4	1	A1.bmp	400	0.28952								6	6	A2.bmp	0.726716	223
4-1-A2.b	341	0.373162		5	1	A1.bmp	357	0.283133		4	1	A1.bmp	0.6102	214
4-2-A1.b	747	0.348168		6	1	A1.bmp	704	0.391003		5	1	A1.bmp	0.528724	484		7	1	A1.bmp	0.636147	306
4-2-A2.b	818	0.274823		4	1	A2.bmp	513	0.236607		6	1	A1.bmp	0.481888	472		7	1	A2.bmp	0.549869	343
4-3-A1.b	505	0.491952		5	1	A2.bmp	400	0.233716		4	1	A2.bmp	0.647834	187		8	1	A1.bmp	0.625	261
4-3-A2.b	562	0.515517		6	1	A2.bmp	784	0.33503		5	1	A2.bmp	0.589547	534		8	1	A2.bmp	0.625551	255
4-4-A1.b	348	0.678967		4	2	A1.bmp	318	0.485437		6	1	A2.bmp	0.449	551		9	1	A1.bmp	0.364879	604
4-4-A2.b	361	0.666359		5	2	A1.bmp	346	0.425249		4	2	A1.bmp	0.674129	262		9	1	A2.bmp	0.643527	190
4-5-A1.b	320	0.692308		6	2	A1.bmp	841	0.446346		5	2	A1.bmp	0.515643	418		7	2	A1.bmp	0.675481	270
4-5-A2.b	203	0.758907		4	2	A2.bmp	347	0.445687		6	2	A1.bmp	0.597403	403		7	2	A2.bmp	0.638849	251
4-6-A1.b	243	0.79213		5	2	A2.bmp	362	0.431711		4	2	A2.bmp	0.686003	249		8	2	A1.bmp	0.526839	238
4-6-A2.b	272	0.697778		6	2	A2.bmp	680	0.511494		5	2	A2.bmp	0.669903	374		8	2	A2.bmp	0.412892	337
5-1-A1.b	644	0.445306		4	3	A1.bmp	157	0.705441		6	2	A2.bmp	0.652559	353		9	2	A1.bmp	0.664748	233
5-1-A2.b	651	0.468137		5	3	A1.bmp	190	0.595745		4	3	A1.bmp	0.707293	293		9	2	A2.bmp	0.569849	428
5-2-A1.b	506	0.546188		6	3	A1.bmp	502	0.658736		5	3	A1.bmp	0.633958	332		7	3	A1.bmp	0.711388	185
5-2-A2.b	367	0.604526		4	3	A2.bmp	224	0.645008		6	3	A1.bmp	0.601093	292		7	3	A2.bmp	0.705167	194
5-3-A1.b	411	0.585685		5	3	A2.bmp	239	0.518145		4	3	A2.bmp	0.636542	370		8	3	A1.bmp	0.65764	177
5-3-A2.b	307	0.578297		6	3	A2.bmp	589	0.637984		5	3	A2.bmp	0.65874	287		8	3	A2.bmp	0.639229	206
5-4-A1.b	336	0.745455		4	4	A1.bmp	297	0.566423		6	3	A2.bmp	0.680492	208		9	3	A1.bmp	0.591727	227
5-4-A2.b	259	0.755891		5	4	A1.bmp	165	0.708481		4	4	A1.bmp	0.608737	412		9	3	A2.bmp	0.608392	224
5-5-A1.b	123	0.855803		6	4	A1.bmp	645	0.547686		5	4	A1.bmp	0.668874	350		7	4	A1.bmp	0.671756	258
5-5-A2.b	115	0.879581		4	4	A2.bmp	271	0.577223		6	4	A1.bmp	0	317		7	4	A2.bmp	0.62379	272
5-6-A1.b	216	0.823385		5	4	A2.bmp	200	0.646018		4	4	A2.bmp	0.583728	440		8	4	A1.bmp	0.637097	180
5-6-A2.b	235	0.78733		6	4	A2.bmp	513	0.645718		5	4	A2.bmp	0.603235	417		8	4	A2.bmp	0.550403	223
6-1-A1.b	406	0.518957		4	5	A1.bmp	287	0.612162		6	4	A2.bmp	0	258		9	4	A1.bmp	0.261084	300
6-1-A2.b	323	0.537911		5	5	A1.bmp	298	0.535826		4	5	A1.bmp	0.671789	299		9	4	A2.bmp	0.321348	302
6-2-A1.b	373	0.604873		6	5	A1.bmp	515	0.654362		5	5	A1.bmp	0.697382	289		7	5	A1.bmp	0.555556	324
6-2-A2.b	306	0.600522		4	5	A2.bmp	266	0.5744		6	5	A1.bmp	0.682731	237		7	5	A2.bmp	0.406732	423
6-3-A1.b	303	0.494157		5	5	A2.bmp	255	0.558059		4	5	A2.bmp	0.685649	276		8	5	A1.bmp	0.490046	333
6-3-A2.b	236	0.564576		6	5	A2.bmp	552	0.606557		5	5	A2.bmp	0.636364	380		8	5	A2.bmp	0.458054	323
6-4-A1.b	240	0.704433		4	6	A1.bmp	110	0.741176		6	5	A2.bmp	0.635475	261		9	5	A1.bmp	0.516393	295
6-4-A2.b	151	0.743633		5	6	A1.bmp	135	0.800591		4	6	A1.bmp	0.460568	513		9	5	A2.bmp	0.487663	353
				6	6	A1.bmp	347	0.737519		5	6	A1.bmp	0.807074	180		7	6	A1.bmp	0.74812	201
				4	6	A2.bmp	137	0.676123		6	6	A1.bmp	0.535452	380		7	6	A2.bmp	0.730408	258
4-1-B1.b	227			5	6	A2.bmp	176	0.740413		4	6	A2.bmp	0.416838	568		8	6	A1.bmp	0.495756	297
4-1-B2.b	203			6	6	A2.bmp	347	0.737915		5	6	A2.bmp	0.717494	239		8	6	A2.bmp	0.597744	214
4-2-B1.b	399			4	1	B1.bmp	163			6	6	A2.bmp	0.539638	331		9	6	A1.bmp	0.755039	158
4-2-B2.b	310			4	2	B1.bmp	300			4	1	B1.bmp		335		9	6	A2.bmp	0.777603	141
4-3-B1.b	489			4	3	B1.bmp	376			4	2	B1.bmp		542
4-3-B2.b	598			4	4	B1.bmp	388			4	3	B1.bmp		708
4-4-B1.b	736			4	5	B1.bmp	453			4	4	B1.bmp		641
4-4-B2.b	721			4	6	B1.bmp	315			4	5	B1.bmp		612
4-5-B1.b	720			5	1	B1.bmp	141			4	6	B1.bmp		438
4-5-B2.b	639			5	2	B1.bmp	256			5	1	B1.bmp		543
4-6-B1.b	926			5	3	B1.bmp	280			5	2	B1.bmp		445
4-6-B2.b	628			5	4	B1.bmp	401			5	3	B1.bmp		575
5-1-B1.b	517			5	5	B1.bmp	344			5	4	B1.bmp		707
5-1-B2.b	573			5	6	B1.bmp	542			5	5	B1.bmp		666
5-2-B1.b	609			6	1	B1.bmp	452			5	6	B1.bmp		753
5-2-B2.b	561			6	2	B1.bmp	678			6	1	B1.bmp		439
5-3-B1.b	581			6	3	B1.bmp	969			6	2	B1.bmp		598
5-3-B2.b	421			6	4	B1.bmp	781			6	3	B1.bmp		440
5-4-B1.b	984			6	5	B1.bmp	975			6	4	B1.bmp
5-4-B2.b	802			6	6	B1.bmp	975			6	5	B1.bmp		510
5-5-B1.b	730			4	1	B2.bmp	159			6	6	B1.bmp		438
5-5-B2.b	840			4	2	B2.bmp	279			4	1	B2.bmp		344
5-6-B1.b	1007			4	3	B2.bmp	407			4	2	B2.bmp		544
5-6-B2.b	870			4	4	B2.bmp	370			4	3	B2.bmp		648
6-1-B1.b	438			4	5	B2.bmp	359			4	4	B2.bmp		617
6-1-B2.b	376			4	6	B2.bmp	286			4	5	B2.bmp		602
6-2-B1.b	571			5	1	B2.bmp	122			4	6	B2.bmp		406
6-2-B2.b	460			5	2	B2.bmp	275			5	1	B2.bmp		767
6-3-B1.b	296			5	3	B2.bmp	257			5	2	B2.bmp		759
6-3-B2.b	306			5	4	B2.bmp	365			5	3	B2.bmp		554
6-4-B1.b	572			5	5	B2.bmp	322			5	4	B2.bmp		634
6-4-B2.b	438			5	6	B2.bmp	502			5	5	B2.bmp		665
				6	1	B2.bmp	395			5	6	B2.bmp		607
7-1-A1.b	383	0.623772		6	2	B2.bmp	712			6	1	B2.bmp		449
7-1-A2.b	403	0.548206		6	3	B2.bmp	1038			6	2	B2.bmp		663
7-2-A1.b	370	0.65709		6	4	B2.bmp	935			6	3	B2.bmp		443
7-2-A2.b	345	0.629431		6	5	B2.bmp	851			6	4	B2.bmp
7-3-A1.b	411	0.547357		6	6	B2.bmp	977			6	5	B2.bmp		455
7-3-A2.b	428	0.523915								6	6	B2.bmp		388
7-4-A1.b	354	0.681368		7	1	A1.bmp	0.339244
7-4-A2.b	270	0		8	1	A1.bmp	0.392725
7-5-A1.b	274	0.724346		9	1	A1.bmp	0.469333			7	1	A1.bmp	0.387211	345
7-5-A2.b	258	0.75452		7	1	A2.bmp	0.354497			8	1	A1.bmp	0.480808	257
7-6-A1.b	311	0		8	1	A2.bmp	0.482699			9	1	A1.bmp	0.207463	531
7-6-A2.b	244	0		9	1	A2.bmp	0.46817			7	1	A2.bmp	0.296937	528
8-1-A1.b	747	0.297272		7	2	A1.bmp	0.435593			8	1	A2.bmp	0.442043	284
8-1-A2.b	718	0.295388		8	2	A1.bmp	0.470644			9	1	A2.bmp	0.303509	397
8-2-A1.b	460	0.580292		9	2	A1.bmp	0.531943			7	2	A1.bmp	0.432574	324
8-2-A2.b	581	0.545027		7	2	A2.bmp	0.355177			8	2	A1.bmp	0.363636	245
8-3-A1.b	318	0.612195		8	2	A2.bmp	0.410433			9	2	A1.bmp	0.445026	318
8-3-A2.b	70	0.87037		9	2	A2.bmp	0.699593			7	2	A2.bmp	0.419469	328
8-4-A1.b	414	0.653556		7	3	A1.bmp	0.431046			8	2	A2.bmp	0.328704	290
8-4-A2.b	268	0.74717		8	3	A1.bmp	0.486074			9	2	A2.bmp	0.390649	404
8-5-A1.b	242	0.819268		9	3	A1.bmp	0.632469			7	3	A1.bmp	0.485994	367
8-5-A2.b	218	0.809107		7	3	A2.bmp	0.359357			8	3	A1.bmp	0.508982	164
8-6-A1.b	248	0.730727		8	3	A2.bmp	0.441745			9	3	A1.bmp	0.475124	211
8-6-A2.b	203	0.762016		9	3	A2.bmp	0.673988			7	3	A2.bmp	0.3821	359
9-1-A1.b	770	0.338488		7	4	A1.bmp	0.504492			8	3	A2.bmp	0.457143	190
9-1-A2.b	821	0.340562		8	4	A1.bmp	0.567127			9	3	A2.bmp	0.489083	234
9-2-A1.b	470	0.596567		9	4	A1.bmp	0.595455			7	4	A1.bmp	0.435275	349
9-2-A2.b	562	0.544201		7	4	A2.bmp	0.529412			8	4	A1.bmp	0.376206	194
9-3-A1.b	352	0.739452		8	4	A2.bmp	0.636057			9	4	A1.bmp	0.319846	353
9-3-A2.b	338	0.731959		9	4	A2.bmp	0.584027			7	4	A2.bmp	0.311644	402
9-4-A1.b	352	0.671028		7	5	A1.bmp	0.522232			8	4	A2.bmp	0.449086	211
9-4-A2.b	300	0.70902		8	5	A1.bmp	0.614652			9	4	A2.bmp	0.317647	348
9-5-A1.b	361	0.685266		9	5	A1.bmp	0.544304			7	5	A1.bmp	0.442225	391
9-5-A2.b	308	0.713755		7	5	A2.bmp	0.453597			8	5	A1.bmp	0.281818	316
9-6-A1.b	357	0.646184		8	5	A2.bmp	0.527002			9	5	A1.bmp	0.508065	305
9-6-A2.b	299	0.704253		9	5	A2.bmp	0.547794			7	5	A2.bmp	0.359511	367
				7	6	A1.bmp	0.53997			8	5	A2.bmp	0.377193	284
7-1-B1.b	635			8	6	A1.bmp	0.541726			9	5	A2.bmp	0.478873	296
7-1-B2.b	489			9	6	A1.bmp	0.599162			7	6	A1.bmp	0.368421	432
7-2-B1.b	709			7	6	A2.bmp	0.502183			8	6	A1.bmp	0.350195	334
7-2-B2.b	586			8	6	A2.bmp	0.546267			9	6	A1.bmp	0.501969	253
7-3-B1.b	497			9	6	A2.bmp	0.591153			7	6	A2.bmp	0.310861	368
7-3-B2.b	471			7	1	B1.bmp				8	6	A2.bmp	0.392079	307
7-4-B1.b	757			7	2	B1.bmp				9	6	A2.bmp	0.476285	265
				7	3	B1.bmp				7	1	B1.bmp		218
7-5-B1.b	720			7	4	B1.bmp				7	2	B1.bmp		247
7-5-B2.b	793			7	5	B1.bmp				7	3	B1.bmp		347
				7	6	B1.bmp				7	4	B1.bmp		269
				8	1	B1.bmp				7	5	B1.bmp		310
8-1-B1.b	316			8	2	B1.bmp				7	6	B1.bmp		252
8-1-B2.b	301			8	3	B1.bmp				8	1	B1.bmp		238
8-2-B1.b	636			8	4	B1.bmp				8	2	B1.bmp		140
8-2-B2.b	696			8	5	B1.bmp				8	3	B1.bmp		170
8-3-B1.b	502			8	6	B1.bmp				8	4	B1.bmp		117
8-3-B2.b	470			9	1	B1.bmp				8	5	B1.bmp		124
8-4-B1.b	781			9	2	B1.bmp				8	6	B1.bmp		180
8-4-B2.b	792			9	3	B1.bmp				9	1	B1.bmp		139
8-5-B1.b	1097			9	4	B1.bmp				9	2	B1.bmp		255
8-5-B2.b	924			9	5	B1.bmp				9	3	B1.bmp		191
8-6-B1.b	673			9	6	B1.bmp				9	4	B1.bmp		166
8-6-B2.b	650			7	1	B2.bmp				9	5	B1.bmp		315
9-1-B1.b	394			7	2	B2.bmp				9	6	B1.bmp		255
9-1-B2.b	424			7	3	B2.bmp				7	1	B2.bmp		223
9-2-B1.b	695			7	4	B2.bmp				7	2	B2.bmp		237
9-2-B2.b	671			7	5	B2.bmp				7	3	B2.bmp		222
9-3-B1.b	999			7	6	B2.bmp				7	4	B2.bmp		182
9-3-B2.b	923			8	1	B2.bmp				7	5	B2.bmp		206
9-4-B1.b	718			8	2	B2.bmp				7	6	B2.bmp		166
9-4-B2.b	731			8	3	B2.bmp				8	1	B2.bmp		225
9-5-B1.b	786			8	4	B2.bmp				8	2	B2.bmp		142
9-5-B2.b	768			8	5	B2.bmp				8	3	B2.bmp		160
9-6-B1.b	652			8	6	B2.bmp				8	4	B2.bmp		172
9-6-B2.b	712			9	1	B2.bmp				8	5	B2.bmp		172
				9	2	B2.bmp				8	6	B2.bmp		198
				9	3	B2.bmp				9	1	B2.bmp		173
				9	4	B2.bmp				9	2	B2.bmp		259
				9	5	B2.bmp				9	3	B2.bmp		224
				9	6	B2.bmp				9	4	B2.bmp		162
										9	5	B2.bmp		272
										9	6	B2.bmp		241

IV.Screening materials for biofilm attachment

Name				Count
1	1	A1.bmp	0.415525	128
1	2	A1.bmp	0.175824	150
1	3	A1.bmp	0.23125	123
1	1	A2.bmp	0.431694	104
1	2	A2.bmp	0.242647	103
1	3	A2.bmp	0.368056	91
2	1	A1.bmp	0.195946	119
2	2	A1.bmp	0.260638	139
2	3	A1.bmp	0.349754	132
2	1	A2.bmp	0.161017	99
2	2	A2.bmp	0.257669	121
2	3	A2.bmp	0.315217	126
3	1	A1.bmp	0.112963	479
3	2	A1.bmp	0.331776	143
3	3	A1.bmp	0.358974	175
3	1	A2.bmp	0.085106	473
3	2	A2.bmp	0.373832	134
3	3	A2.bmp	0.243402	258
4	1	A1.bmp	0.237069	177
4	2	A1.bmp	0.420765	106
4	3	A1.bmp	0.504505	110
4	1	A2.bmp	0.305419	141
4	2	A2.bmp	0.357143	108
4	3	A2.bmp	0.569536	65
5	1	A1.bmp	0.358744	143
5	2	A1.bmp	0.438095	118
5	3	A1.bmp	0.421053	143
5	1	A2.bmp	0.333333	162
5	2	A2.bmp	0.330189	142
5	3	A2.bmp	0.296875	225
6	1	A1.bmp	0.435294	96
6	2	A1.bmp	0.449664	82
6	3	A1.bmp	0.5	50
6	1	A2.bmp	0.38835	126
6	2	A2.bmp	0.601266	63
6	3	A2.bmp	0.581818	46
1	1	B1.bmp		91
1	2	B1.bmp		32
1	3	B1.bmp		37
2	1	B1.bmp		29
2	2	B1.bmp		49
2	3	B1.bmp		71
3	1	B1.bmp		61
3	2	B1.bmp		71
3	3	B1.bmp		98
4	1	B1.bmp		55
4	2	B1.bmp		77
4	3	B1.bmp		112
5	1	B1.bmp		80
5	2	B1.bmp		92
5	3	B1.bmp		104
6	1	B1.bmp		74
6	2	B1.bmp		67
6	3	B1.bmp		50
1	1	B2.bmp		79
1	2	B2.bmp		33
1	3	B2.bmp		53
2	1	B2.bmp		19
2	2	B2.bmp		42
2	3	B2.bmp		58
3	1	B2.bmp		44
3	2	B2.bmp		80
3	3	B2.bmp		83
4	1	B2.bmp		62
4	2	B2.bmp		60
4	3	B2.bmp		86
5	1	B2.bmp		81
5	2	B2.bmp		70
5	3	B2.bmp		95
6	1	B2.bmp		80
6	2	B2.bmp		95
6	3	B2.bmp		64

V.Verification of effects of our parts

Name				Count
1	1	A1.bmp	0.330935	186
1	2	A1.bmp	0.259912	168
1	3	A1.bmp	0.310924	164
1	1	A2.bmp	0.260223	199
1	2	A2.bmp	0.18239	260
1	3	A2.bmp	0.268293	180
2	1	A1.bmp	0.244541	173
2	2	A1.bmp	0.19084	106
2	3	A1.bmp	0.546512	39
2	1	A2.bmp	0.215768	189
2	2	A2.bmp	0.232394	109
2	3	A2.bmp	0.597938	39
3	1	A1.bmp	0.590164	50
3	2	A1.bmp	0.359551	57
3	3	A1.bmp	0.280576	100
3	1	A2.bmp	0.504065	61
3	2	A2.bmp	0.411111	53
3	3	A2.bmp	0.178218	83
4	1	A1.bmp	0.392157	124
4	2	A1.bmp	0.57672	80
4	3	A1.bmp	0.536585	57
4	1	A2.bmp	0.406977	102
4	2	A2.bmp	0.433121	89
4	3	A2.bmp	0.415663	97
5	1	A1.bmp	0.383784	114
5	2	A1.bmp	0.379747	98
5	3	A1.bmp	0.438819	133
5	1	A2.bmp	0.370166	114
5	2	A2.bmp	0.20625	127
5	3	A2.bmp	0.365079	120
6	1	A1.bmp	0.322835	86
6	2	A1.bmp	0.303797	110
6	3	A1.bmp	0.35	65
6	1	A2.bmp	0.306569	95
6	2	A2.bmp	0.263566	95
6	3	A2.bmp	0.376147	68
7	1	A1.bmp	0.558252	91
7	2	A1.bmp	0.555556	52
7	3	A1.bmp	0.363265	156
7	1	A2.bmp	0.493976	84
7	2	A2.bmp	0.663866	40
7	3	A2.bmp	0.216981	166
8	1	A1.bmp	0.362069	111
8	2	A1.bmp	0.464646	53
8	3	A1.bmp	0.376623	96
8	1	A2.bmp	0.425	92
8	2	A2.bmp	0.541176	39
8	3	A2.bmp	0.458333	91
1	1	B1.bmp		92
1	2	B1.bmp		59
1	3	B1.bmp		74
2	1	B1.bmp		56
2	2	B1.bmp		25
2	3	B1.bmp		47
3	1	B1.bmp		72
3	2	B1.bmp		32
3	3	B1.bmp		39
4	1	B1.bmp		80
4	2	B1.bmp		109
4	3	B1.bmp		66
5	1	B1.bmp		71
5	2	B1.bmp		60
5	3	B1.bmp		104
6	1	B1.bmp		41
6	2	B1.bmp		48
6	3	B1.bmp		35
7	1	B1.bmp		115
7	2	B1.bmp		65
7	3	B1.bmp		89
8	1	B1.bmp		63
8	2	B1.bmp		46
8	3	B1.bmp		58
1	1	B2.bmp		70
1	2	B2.bmp		58
1	3	B2.bmp		66
2	1	B2.bmp		52
2	2	B2.bmp		33
2	3	B2.bmp		58
3	1	B2.bmp		62
3	2	B2.bmp		37
3	3	B2.bmp		18
4	1	B2.bmp		70
4	2	B2.bmp		68
4	3	B2.bmp		69
5	1	B2.bmp		67
5	2	B2.bmp		33
5	3	B2.bmp		69
6	1	B2.bmp		42
6	2	B2.bmp		34
6	3	B2.bmp		41
7	1	B2.bmp		82
7	2	B2.bmp		79
7	3	B2.bmp		46
8	1	B2.bmp		68
8	2	B2.bmp		46
8	3	B2.bmp		77