Team:Bio Without Borders/Notebook

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Notebook

Week 1 (June 4-8)

•Made competent cells

Week 2 (June 11-15)

•Testing competent cells using iGem DNA transformation samples.

• Tested to see if they grew better on a freezer block versus an ice bath.

• Ran colony PCR and plated bacteria on antimicrobial plate.

• cPCR, chloramphenicol, and culturing protocols, made agarose gel

• gel electrophoresis for cPCR, nucleic acid purification plasmid

Week 3 (June 18-22)

•Made chloramphenicol LB plates

•Restriction enzyme digest of iGem plasmid PSB1C3 using EcoRI and PstI

•Made digest master mix; ran an ezyme digest of iGem plasmids PSB1A3 and PSB1K3 Performed ligation of

PSB1A3 and PSB1K3; transformed PSB1A3 in competent cells on ampicillin plate