Demonstrate
Gold Medal Criterion #4
XYLOSE AS FEEDSTOCK
✔Successfully constructed an inducible xylose-utilizing module
✔Demonstrated improved growth of E. coli BL21* containing this module in xylose and glucose-xylose mixture
DE NOVO BIOSYNTHESIS
✔Successfully constructed a naringenin-producing plasmid with just a single missing enzyme required for full de novo synthesis
✔Demonstrated production of naringenin from coumaric acid in E. coli Acella and BL21*
LUTEOLIN
✔Successfully constructed chemically-inducible and light inducible luteolin-producing plasmids
✔Demonstrated production of luteolin from naringenin in E. coli BL21*
✔Characterized Bba_ (F3’H), Bba (FNS), Bba (pBAD-FNS)
BLUE LIGHT REPRESSIBLE SYSTEM
✔Improved characterization of EL222 blue light repressible promoter, PBLrep (BBa_)
✔Improved characterization of EL222 blue light inducible promoter, PBLind (BBa_)
✔Demonstrated blue light repression of luteolin production
STRESS REPORTER
✔Successfully constructed a stress reporter module
✔Characterized the burden responsive promoter, PhtpG1
✔Demonstrated stress induced by naringenin- and luteolin-producing plasmids
CELL-MACHINE INTERFACE
✔Designed and built devices which help characterize optogenetic circuits in petri dishes and 250 ml conical flasks
✔Designed and built a 500 ml working volume benchtop optogenetic bioreactor, which comprises a peristaltic pump, 2-in-1 OD and fluorescence sensor, and fermentation chamber
✔Designed and implemented a feedback control system to control the optogenetic bioreactor