Team:AHUT China/protocol
Protocols
LB Medium（1L）： |
|
tryptone |
10g |
yeast extract |
5g |
NaCl |
10g |
ddH2O |
|
Adjustable pH: 1l plus 200μl,5mol/L NaOH pH to 7.0 |
|
LB Srednje（1L solid）
tryptone |
10g |
yeast extract |
5g |
NaCl |
10g |
agar |
15g |
ddH2O |
|
Adjustable pH: 1l plus 200μl,5mol/L NaOH pH to 7.0 |
|
50*TAE（1L） |
|
ddH2O |
500ml |
Tris buffer solution |
242g |
0.5mol·l-1 EDTA |
100ml |
Glacial acetic acid |
57.1ml |
ddH2O |
|
0.25 % dye(1L) |
|
G-250 |
100mg |
90%ethanol |
50ml |
85% pHospHoric acid |
10ml |
ddH2O |
|
10 % seperate adhesive(10ml) |
|
distilled water |
1.9ml |
30%Arc-Bis |
1.7ml |
1.5M Tris-HCl pH 8.8 |
1.3ml |
10%SDS |