Team:Uppsala/Reporter System

Design Protocols Experiment Result Notebook

Feces Analysis

The purpose of this experiment was to determine if fluorescent eukaryotic chromoproteins could be used as a reporter in horse manure. One of these proteins, amilGFP (green fluorescent protein) was chosen because it gave us a opportunity to work with a reporter system developed by earlier iGEM teams of Uppsala, namely those of 2011, 2012 and 2013. To test whether amilGFP was viable or not as a reporter system we decided to run some tests to see if amilGFP could be detected in horse manure, and later, to what extent using a plate reader (fluorescence spectrophotometer) with varying concentrations of amilGFP in liquid culture in horse manure.

Design Protocols Experiment Result Notebook

Refereces

[1] Bowen R. Microbial Life in the Digestive Tract. online: http://www.vivo.colostate.edu/hbooks/pathphys/digestion/basics/gi_bugs.html. Accessed October 12, 2018.

[2] Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology | Journal of Biological Engineering | Full Text. online: https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0100-0. Accessed October 12, 2018.

[3] Kumagai A, Ando R, Miyatake H, Greimel P, Kobayashi T, Hirabayashi Y, Shimogori T, Miyawaki A. 2013. A Bilirubin-Inducible Fluorescent Protein from Eel Muscle. Cell 153: 1602–1611.

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