InterLab

Just Why?- Manu

Introduction

As a kickoff for our work in the lab, our team decided to take part in this year’s international InterLab Measurement Study, intending to improve measurement tools and to develop a solid and repeatable measurement protocol, making the comparison of measurements carried out in different labs more straightforward. Since being one of the most utilized markers all over the world, the fluorescent protein GFP (Green Fluorescent Protein) was chosen as a measurement marker.

In order to contribute to the 2018 InterLab Measurement study, the participating teams were advised to measure the OD600 and the fluorescence of 8 different constructs expressed in DH5a E.colis on a plate reader. Additionally, a protocol to calibrate OD600 to colony forming units (CFU) counts, which directly refer to the cell concentration in the culture, was provided.

Materials and Methods

2.1 Materials

Plate Reader: Tecan Infinite M200 Pro

96 well plates (white): Source unknown

DH5a Escherichia Coli

glass test tubes

2.2 Devices

Hier muss eine Tabelle rein

2.3 Workflow Cell Measurements

Hier muss der Workflow rein

Measurements

3.1 Calibration Measurements

All calibration measurements were performed as suggested by the protocol.

3.1.1 OD600 Reference Point– Ludox CL-X