Methods & Data
Overview
In the interest of promoting reproducible science, and enabling others to build off of our work, we have included both the precise methods used as well as the raw data (in csv format) for every experiment mentioned on our wiki. Experiments are split up by the page on which they are mentioned. For experiments performed with flow cytometry, .fcs files for both measurements and calibration beads (Spherotek RCP-30-5A, lot number: AB01) are included as a separate file. For all experiments, unless stated otherwise, the following is true: Circuits used were present in psb1C3, antibiotics were used in the following concentrations [Chloramphenicol: 7.5mg/mL, Kanamycin: 35mg/mL], the media used was M9 minimal media with 0.4% Casamino Acids and 0.4% Glucose (see protocols).
/div>
Heat Inducible System