Team:UPF CRG Barcelona

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Basic Parts

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BASIC PARTS

BBa_K2581014: FadE

The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the acyl-coA dehydrogenase (FadE) gene which converts acyl-coA into enoyl-coA. This part is a twin of the BBa_K861020).

<img src="/assets/T--UPF_CRG_Barcelona--partFadE.svg" style="width: 35%;">

BBa_K2581015: FadL

The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the transmembrane protein FadL. This gene has an essential role in the fatty acid transportation across the cell membrane via a transportation.

<img src="/assets/T--UPF_CRG_Barcelona--partFadL.svg" style="width: 35%;">

Bba_K2581016: FadD

The UPF_CRG_Barcelona iGEM team 2018 has synthesized this part as a result of a pcr amplification from the DH5-alpha E. coli strain. It consists of the coding sequence of the acyl-coA synthetase gene FadD. This gene has an essential role in the fatty acid transportation inside the cell and in acyl-activation mechanism.

<img src="/assets/T--UPF_CRG_Barcelona--partFadD.svg" style="width: 35%;">

BBa_K2581020: pAR

The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pFadBA (BBa_K817002). This new promoter has two binding sites for FadR in comparison with the existing part.

<img src="/assets/T--UPF_CRG_Barcelona--pARbasic.svg" style="width: 35%;">


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