Team:NUS Singapore-A/shadow/Composite Part
OVERVIEW
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LIST OF COMPOSITE PARTS
| Device | Part Number | Usage |
|---|---|---|
| PhtpG1-mRFP | BBa_K2819118 | Stress reporter |
| Positive Control | BBa_I20270 | Promoter MeasKit (J23151) |
EXPERIMENTS
Abs600 |
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Fluorescence |
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CONCLUSION
The results from our experiment seem to indicate that normalizing fluorescence measurements to absolute cell count using the Study’s two methods will not be able to reduce lab-to-lab variability because counting colony-forming units do not return the expected cell concentration, i.e. the cell concentration modeled by the silica beads in Method 1. While both methods cannot be used independently to establish a robust fluorescence measurement system, it may be possible that lab-to-lab variability can be reduced if a different method of normalizing to absolute cell count is devised, replacing Method 1, Method 2, or both.
HEAR OUR STORY
We’re using synthetic biology to change the way the world thinks and functions.
We’re a little disruptive, dangerous and maybe a tad too bold. But the world needs some shaking up every now and then, and we think we’re just the right people for that.
Together we’re going to engineer biology and create something awesome.
CONTACT US
nusigem@gmail.com21 Lower Kent Ridge Rd, Singapore 119077