| CRISPR-Cas Primers | sequence |
|---|
| 588 FW | CTTGAAAACTTACGCTCATCTCTTCGACG |
| 589 RV | CGGACTTAAGAAAATCCAGGATTGTCTTTCC |
| 6006 FW | GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTC |
| 6005 RV | GATCATTTATCTTTCACTGCGGAGAAG |
| F ARO10 tar | TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCAATATACGAACGAAACAATGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC |
| R ARO10 tar | GTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACCATTGTTTCGTTCGTATATTGATCATTTATCTTTCACTGCGGAGAAGTTTCGAACGCCGAAACATGCGCA |
| F AGA2 KO tar | TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCAAAACATACTGTGTGTTTATGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC |
| R AGA2 KO tar | GTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACATAAACACACAGTATGTTTTGATCATTTATCTTTCACTGCGGAGAAGTTTCGAACGCCGAAACATGCGCA |
| F AGA1 OE tar | TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATCTTACGTTCATCTTTGAAAAAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC |
| R AGA1 OE tar | GTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACTTTTTCAAAGATGAACGTAAGATCATTTATCTTTCACTGCGGAGAAGTTTCGAACGCCGAAACATGCGCA |
| CCasR1-RV | CGTGTAATCTGTGACAATTTCGATGCGTACACTGCGGCCCCGAAGTAGCATAGCTTATCTGGATCTGAACCTCGACTTTCTGGATCAGCC |
| CCasR2-FW | AGATAAGCTATGCTACTTCGGGGCCGCAGTGTACGCATCGAAATTGTCACAGATTACACGCGCTCTAGCATGGAACCAAGACCCATGACG |
| CCasR2-RV | TGAAATGTTATTATGTACCTCAGTACGCGCTAACATCCCTGGGTTACGCATCACTACATTGGATCTGAACCTCGACTTTCTGGATCAGCC |
| CCasR3-RV | TGTATCGAGCGATGTACGAATACTCTTGCCGAGTCATAGCAGTGCAGTAGAGCCTGACCCGGATCTGAACCTCGACTTTCTGGATCAGCC |
| CCasR3FW | AATGTAGTGATGCGTAACCCAGGGATGTTAGCGCGTACTGAGGTACATAATAACATTTCACGCTCTAGCATGGAACCAAGACCCATGACG |
| CCasR4-FW | GGGTCAGGCTCTACTGCACTGCTATGACTCGGCAAGAGTATTCGTACATCGCTCGATACACGCTCTAGCATGGAACCAAGACCCATGACG |
| CCasR5-FW | GCTCTCAACAGTCACTTGTGTGCCAAAATATTATACCTACTTCATACATTTTCAATTAAGCGCTCTAGCATGGAACCAAGACCCATGACG |
| CCasR5-RV | TAATCTTACACTAGTTTATAAGTGTCACGAACGATGAAATGGAAACTTGGGGGGGAAGGCGGATCTGAACCTCGACTTTCTGGATCAGCC |
| CCasR1-FW | ACAAGTTGACGCGACTTCTGTAAAGTTTATTTACAAGATAACAAAGAAACTCCCTTAAGCCGCTCTAGCATGGAACCAAGACCCATGACG |
| CCasR4-RV | TGTTAATGAACAGAAAACGAACAATTGGTAGCAGTGTTTTATAATTGCGCCCACAAGTTTGGATCTGAACCTCGACTTTCTGGATCAGCC |
| CCasAga1-FW | GCGCAGAATAATGATCTATCATCAATAATTATCGTGAACTTTTCCAGTATTTACGTTCATGCATGGAACCAAGACCCATGA |
| CCasAga1-RV | ATTAGTTAATCCCAACAATATTGTGAACAGGTAGGTAAAATGAGCGAAAGATAATGTCATACGAATGTACTAATTAAAAGATCAGAAAA |
| Cellulosome primers | sequence |
|---|
| BGLI-fragA-fw | TAGTAATGCAGACACTTGCG |
| BGLI-fragA-rv | CAACAGCGGTCTACTGAATC |
| BGLI-fragB-fw | CACTAGCTCAGATTCAGTAGACC |
| BGLI-fragB-rv | CGAGATGGACCGCAAG |
| BGLI1-FW | TTTGGATCCGTCTAGATGAA |
| BGL1-RV | AAACTCGAGTACTAGTATTC |
| BGL1A-FW | TTTGGATCCGTCTAGATGAGATTTCCTTCAATTTTTAC |
| BGL1A-RV | AAACTCGAGTACTAGTACTAATAAGGTAGGTGGGGTA |
| CBHII1-FW | TTTGGATCCGTCTAGATGAA |
| CBHII1-RV | AAACTCGAGTACTAGTAACT |
| CIPA3A-FW | TTTGGATCCGTCTAGATGCA |
| CIPA3A-RV | AAAGTCGACCTCGAGTACTA |
| CIPA3B-FW | TTTGGATCCGTCTAGATGCAGTTACTTCGCTGTTT |
| CIPA3B-RV | AAACTCGAGTACTAGTATTAGTGATGGTGATGGTGATGTTCGAAGGGCCCATTCGAAT |
| CIPA3C-RV | AAAGTCGACCTCGAGTATCA |
| EGII1-FW | TTTGGATCCGTCTAGATGAA |
| EGII1-RV | AAACTCGAGTACTAGTAGAC |
| Bio-His-EG-FW1 | TATTTGAATTCGCGGCCGCTTCTAGATGCATCATCACCATCACCATG |
| Bio-His-EG-RV1 | TATTTCTGCAGCGGCCGCTACTAGTATTACTTTCTTGCGAGACACGAG |
| Bio-ss1-HEGII-FW1 | TATTTGAATTCGCGGCCGCTTCTAGATGAAGGTTTTGATTGTCTTG |
| Int-ss1-HEGII-FW1 | TTTGGATCCGTCTAGATGATGAAGGTTTTGATTGTCTTG |
| Int-ss1-HEGII-RV1 | AAACTCGAGTACTAGTATTACTTTCTTGCGAGACACGAGC |
| PAL2 primers | sequence |
|---|
| PAL2A-FW | TTTGGATCCGTCTAGATGGA |
| PAL2A-RV | AAACTCGAGTACTAGTATTAGCAAATCGGAATCGGAGCTC |
| PAL2B-FW | TTTGGATCCATGCATCACCATCACCATCACGATCAAATCGAAGCAATGTT |
| PAL2B-RV | AAACTCGAGTACTAGTATTAATGGTGATGGTGATGGTGGCAAATCGGAATCGGAGC |
| PAL2C-FW | TTTGGATCCGTCTAGATGGATCAAATCGAAGCAAT |
| PAL2.1-FW | CACTAGCTCAGATTCAGTAG |
| PAL2.1-RV | AAACCATGGATAGCCTTGTT |
| PAL2.2-RV | TTAGCAGATTGGGATTGGGG |
| PAL2D-FW | ATATACCAGCATTTAAGAACCTAATGAGTTCTGTTTGTAATGCG |
| PAL2D-RV | AGGTTCTTAAATGCTGGTATATTCGGAAACACGAAGGAGA |
| Pal2F-fw | TTTGGATCCGTCTAGATGGATCAAATAGAAGCAAT |
| Pal2F-rv | AAACTCGAGTTAGTGATGGTGATGGTGATGGCAGATTGGGATTGGGGCAC |
| Pal2G-fw | TTTGGATCCATGCATCACCATCACCATCACATGGATCAAATAGAAGCAAT |
| Pal2G-rv | AAACTCGAGTACTAGTATTAGCAGATTGGGATTGGGG |
| PAL2-fragA-fw | TTTGGATCCGATGGATCAAATAGAAGCAATGTTG |
| PAL2-fragA-rv | AAACCATGGATAGCCTTGTTTC |
| PAL2-fragB-fw | ACTAGCTCAGATTCAGTAGACC |
| PAL2-fragB-rv | AAACTCGAGTTAGCAGATTGGGATTGGGG |
| P T7 | GACCATCACACCACTGAAGACTGCGG |
| R T7 | GTGATCATGAATTAATAAAAGTGTTCGC |
| PAL2-BB-fix-fw | TTAATGAATTCGCGGCCGCTTCTAGATGGACCAAATTGAGGCGATG |
| PAL2-BB-fix-rv | TTAATCTGCAGCGGCCGCTACTAGTACAGTTAGCAAATCGGAATCG |
| PAL2-BB-fw | GAATTCGCGGCCGCTTCTAGATGGACCAAATTGAGGCGATG |
| PAL2-BB-rv | CTGCAGCGGCCGCTACTAGTACAGTTAGCAAATCGGAATCG |
| Synthetic DNA sequences | Source |
|---|
| ss1-V5-EGII-DocS | "Ordered from IDTA, designed based on Wen et al. 2010" |
| ss1-FLAG-CBHI-DocS | "Ordered from IDTA, designed based on Wen et al. 2010" |
| ss1-myc-BGLI-DocA part1 | "Ordered from IDTA, designed based on Wen et al. 2010" |
| ss1-myc-BGLI-DocA part2 | "Ordered from IDTA, designed based on Wen et al. 2010" |
| AGA2-CIPA3-His part1 | "Ordered from IDTA, designed based on Wen et al. 2010" |
| AGA2-CIPA3-His part2 | "Ordered from IDTA, designed based on Wen et al. 2010" | |
| synthetic PAL2 gene part1 | "Ordered from IDTA, designed based on McKenna, 2014" |
| synthetic PAL2 gene part2 | "Ordered from IDTA, designed based on McKenna, 2014" |
