Team:JNFLS/InterLab

We are willing to participate in iGEM 2018 InterLab Study, thanks to iGEM HQ provided us this optunity to share our results with many iGEMers. For improving reproducibility, we use plate readers to take measurements of fluorescence and absorbance in accordance with the requirements.

Before we begin our experiments, we had known all the informations about our plate reader, which are provided when we submitted our data to iGEM HQ. And we prepared all of the supplies and reagents used in the experiments.

1.Calibrations

For all of the calibration measurements, we used the same plates and volumes, we also used the same settings, such as filters, excitation and emission wavelengths, temperatures, etc.

1.1 Calibration 1: OD600 Reference point

According to the LUDOX Protocol, we used LUDOX CL-X (45% colloidal silica suspension) as a single point reference to obtain a conversion factor to transform the absorbance (Abs600) data from the plate reader into a comparable OD600 measurement obtained in a spectrophotometer. NOTE: we did not find an automatic path length correction on our instrument. After finish all the processed, we obtained our correction factor to convert measured Abs600 to OD600, it is 6.222, so all the Abs600 readings using this instrument with the same settings and volume were converted to OD600 by multiplying by 6.222.