Team:Munich/chibiobrick1.html

Forming oligodimers from single-strand DNA chi6 sequences

2018/06/01
Participants: Dominic
Protocol: Oligodimerization

Assembling pSB1C3_Chi6 with A3 Assembly

2018/06/04
Participants: Dominic
Protocol: Restrition digest, Ligation, Chemical transformation, Mini prep, Sequencing
Notes: digest with XbaI, SpeI
transformation of Dh5a
Results: no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment.

Redo: Assembling pSB1C3_Chi6 with A3 Assembly

2018/06/06 - 2018/06/26
Participants: Dominic
Protocol: Restrition digest, PCR purification, Ligation, Chemical Transformation, PCR, Agarose gel
Notes: digest with XbaI, SpeI
transformation of Dh5a
Results: No bands on the Gel. Colonies on the plate resulted from contamination.