SUMMARY OF WET LAB RESULTS UC DAVIS 2012 Confirmed cutinase activity using PNPB esterase assay Engineered E. coli ethylene glycol metabolism with directed evolution BAU-Indonesia 2012 Isolation of cutinase gene from nature with primers TU Darmstadt 2012 Surface display of cutinase on E. coli Attempted TPA transport into E. coli, further research required Expressed all TPH enzymes, did not attempt to measure activity Confirmed anaerobic conversion of PCA via AroY and XylE enzymes Imperial_College 2013 Produced P3HB bioplastic from mixed waste containing at least some PET METU_Turkey 2014 Reduced catechol to pyruvate ITB-Indonesia 2014 LC cutinatse activity confimed with SEM, PNPB Pasteur Paris 2015 PNPB assay to confirm activity of esterase EST13 Fluorescent detection of TPA can not be accomplished when in LB broth. Harvard 2016 Petase function confirmed with PNPB Bacteria produced electric current when supplied with unspecified quantity of TPA ASIJ Tokyo 2016 Attempt at detecting PET degradation by mass change failed UoA_NewZealand 2016 Assembled PETase part with His tag BGU-Israel 2016 PNPB and EM to confirm LC cutinase activity P. putida can grow on PCA as sole carbon source, but not TPA. E. coli expressing LC-cutinase with pelB leader sequence grew on M9 plates with PET as sole carbon source. Expected to be due to consumption of ethylene glycol from PET degradation. Unable to determine enzyme efficiency based on growth due to heterogeneity in PET distribution Measured fluorescence of TPA on plates, unable to quantify LC cutinase activity. TJUSLS China 2016 HPLC detection of MHET to confirm PETase activity in varying conditions Surface display of PETase in E. coli Tianjin 2016 EM confirmation of PETase activity of PET film degradation Multispectral scanning quantified PETase products for cell free system Baltimore BioCrew 2016 Planned to weigh PET degradation, no results UESTC China 2016 SEM and PNPB to confirm PETase activity Possible detection of TPA by UV vis (higher absorbance across spectrum) AUC_Turkey 2016 Withdrawn ITB-Indonesia 2017 PNPB and SEM to confirm PETase activity Successful biofilm formation on PET, but biofilm matrix hampered PETase activity. Baltimore BioCrew 2017 Fluorescine diacetate hydrolysis assay to confirm PETase and MHETase hydrolytic activity BOKU-Vienna 2017 Discussion of a possible method for directed evolution of PETase by culturing cells on PET film that fluoresces when degraded If you see this then 2018 results from other teams have not been posted in time to show them here before the wiki freeze.

Team:UMaryland/PETaseIntro

History

iGEM teams pursuing PET related projects