Team:Munich/assembly3.html

T7 and 3S Phage Assembly in self-produced cell-extract

2018/09/21- 2018/09/22
Participants: Brigit Tunaj, Sophie v. Schönberg
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: T7 DNA: ca. 500 ng, 3S Phage: 330 ng Self-produced extract: E10
Results: T7 Phage: 1,6*10^8 PFU/mL 3S: did not work out

T7 Phage Assembly in self-produced cell-extract

2018/09/21- 2018/09/22
Participants: Brigit Tunaj, Sophie v. Schönberg
Protocol: PhageT7AssemblyTXTL, Agar overlay plaque assay
Notes: T7 DNA: ca. 500 ng,
Results: T7 Phage in E10 Extract: 3*10^4 PFU/mL T7 Phage in A8 Extract: 2*10^4 PFU/mL