T7 and 3S Phage Assembly in self-produced cell-extract
2018/09/21- 2018/09/22Participants: | Brigit Tunaj, Sophie v. Schönberg |
Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
Notes: | T7 DNA: ca. 500 ng, 3S Phage: 330 ng Self-produced extract: E10 |
Results: | T7 Phage: 1,6*10^8 PFU/mL 3S: did not work out |
T7 Phage Assembly in self-produced cell-extract
2018/09/21- 2018/09/22Participants: | Brigit Tunaj, Sophie v. Schönberg |
Protocol: | PhageT7AssemblyTXTL, Agar overlay plaque assay |
Notes: | T7 DNA: ca. 500 ng, |
Results: | T7 Phage in E10 Extract: 3*10^4 PFU/mL T7 Phage in A8 Extract: 2*10^4 PFU/mL |