Team:Munich/engineering1.html
Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/04
| Participants: | Dominic Schwarz |
| Protocol: |
<a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical Transformation</a> |
| Notes: | inoculate pRED at 30°C because of temperature sensitive promoter |
| Results: | no colonies. We decided to redo the experiment with another pRED plasmid from PD Dr. Sandra Sagredo from the Simmel lab. |
Redo: Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/08
| Participants: | Dominic Schwarz |
| Protocol: |
<a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical Transformation</a> |
| Notes: | we used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN |
| Results: | no colonies. We decided to redo the experiment by electroporation. |
Redo:Transforming E. Coli Rosetta for pRED/ET engineering
2018/06/12
| Participants: | Dominic Schwarz |
| Protocol: |
<a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">Restrition digest</a> |
| Notes: | we used a different pRED/ET plasmid from PD Dr. Sandra Sagredo from the ZNN |
| Results: | no colonies. pRED/ET engineering seems to not work in Rosetta. we postponed this experiment to do it with PD Dr. Lassak at the LMU again. |