Team:Munich/preparingge.html

Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering

2018/08/28

Participants: Dominic Schwarz
Protocol:

<a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">ELectrocompetent transformation</a>

Notes: inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.
Results: ~200 colonies