Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering
2018/08/28
Participants: | Dominic Schwarz |
Protocol: |
<a href="https://static.igem.org/mediawiki/2018/c/ca/T--Munich--WL1_Electrocompetent_transformation.pdf" target="_blank">ELectrocompetent transformation</a> |
Notes: | inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid. |
Results: | ~200 colonies |