Team:Munich/recbcdtev2.html


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assemble pSB1C3_RecBCD-TEV

Participants: Enikö Baligács
Protocol:

<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>, <a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, <a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical transformation</a>

Notes: RecB_TEV_rv & RecC_TEV_fw: expected: 3,7 kb

RecC_TEV_rv & RecB_TEV_fw: expected: 7,6 kb

Results: ??