Team:Munich/recbiobricks.html
Assembling biobrick for RecB, C, D
2018/08/31
| Participants: | Enikö Baligács |
| Protocol: |
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a>, <a href="https://international.neb.com/protocols/2015/11/23/monarch-dna-gel-extraction-kit-protocol-t1020" target="_blank">Gel extraction</a>, <a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, <a href="https://static.igem.org/mediawiki/2018/a/a5/T--Munich--WL1_Chemical_transformation.pdf" target="_blank">Chemical transformation</a>, <a href="https://international.neb.com/protocols/2015/11/20/monarch-plasmid-dna-miniprep-kit-protocol-t1010" target="_blank">Mini Prep</a> |
| Notes: | RecD_BB_EcoRI_fw & RecD_BB_SpeI_rv (3,5 kb) RecB_BB_EcoRI_fw & RecB_BB_EcoRi_rv (1,8 kb) |
| Results: | PIC only one worked, but somehow all were ligated :D stuff missing? |
TestPCR for Biobricks of RecB, C, D
2018/09/03
| Participants: | Enikö Baligács |
| Protocol: |
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a> |
| Notes: | Primers: VF2 & VR |
| Results: | ? |
Removing PstI restriction site from pSB1C3_RecB
2018/09/03
| Participants: | Enikö Baligács |
| Protocol: |
<a href="https://static.igem.org/mediawiki/2018/3/3c/T--Munich--WL1_PCR.pdf" target="_blank">PCR</a>, <a href="https://international.neb.com/protocols/2015/11/23/monarch-pcr-and-dna-cleanup-kit-protocol" target="_blank">PCR purification</a>, <a href="https://static.igem.org/mediawiki/2018/1/1a/T--Munich--WL1_Ligation.pdf" target="_blank">Ligation</a>, <a href="https://international.neb.com/protocols/2012/12/11/gibson-assembly-protocol-e5510" target="_blank">Gibson assembly</a>, <a href="https://static.igem.org/mediawiki/2018/f/fc/T--Munich--Transformation01.pdf" target="_blank">Transformation</a> |
| Notes: | because of time restraints, this experiment was done using ligation and gibson assembly approaches in parallel PCR: RecB_BB_PstI-remove-Lig
Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv
Templ: RecB_BioBrick Plasmid
AT: 72° ET: 11 min
|
| Results: | ? |