Team:NUS Singapore-Sci/Parts
Parts
Basic Parts
| Part Name | Component | Description |
|---|---|---|
| BBa_K2807000 | rAPOBEC1 | Rat APOBEC1. Used to perform the C-to-U conversion. |
| BBa_K2807001 | dPspCas13b | "Dead" Cas13 from Prevotella sp.. Used to target specific mRNA strands. |
| BBa_K2807002 | PspCas13b | Cas13 from Prevotella sp.. Used to target and cut specific mRNA strands. |
| BBa_K2807005 | eGFP | Consistutively expressed GFP reporter. Used in the Reporter system. |
| BBa_K2807006 | mCherry | Red flurophore protein. Used in the Reporter system. |
| BBa_K2807007 | T2A | Self cleavage peptide. Used in the Reporter system. |
| BBa_K2807008 | GLB1 | Human lysosomal acid beta galactosidase. Used in the GLB reporter system. |
| BBa_K2807009 | eGFP mutant reporter | eGFP with a mutated start codon (ATG -> ACG). |
| BBa_K2807010 | GLB1 CCG mutant | Non-functional beta galactosidase with mutation of I389 to P389. |
| BBa_K2807011 | GLB1 TCT mutant | Non-functional beta galactosidase with mutation of F107 to L107. |
Composite Parts
| Part Name | Component | Description |
|---|---|---|
| BBa_K2807003 | rAPOBEC1-XTEN-dPspCas13b | The completed Editor system. |
| BBa_K2807012 | eGFP-T2A-mCherry | The completed Dual-Reporter system. |
| BBa_K2807013 | eGFP-T2A-mCherry mutant | The completed Dual-Reporter system, mutated for BioBric competency. |
| BBa_K2807014 | eGFP-GLB1 WT | The completed GLB-Reporter system. |
| BBa_K2807015 | eGFP-GLB1 CCG mutant | |
| BBa_K2807016 | eGFP-GLB1 TCT mutant |