Team:Munich/qpcr1.html
Forming oligodimers from single-strand DNA chi6 sequences
2018/05/28| Participants: | Nils O'Brien |
| Protocol: | Gel extraction, NEB Q5 High-Fidelity DNA Polymerase Kit #M0492S, NEB Taq Polymerase Kit (nummer) | Notes: |
T7 Phage: TP01_gene (qPCR_Fwd_T7p01, qPCR_Rvd_T7p01) TP07_gene (qPCR_Fwd_T7p07, qPCR_Rvd_T7p07) --> did not work T7p29_gene (qPCR_Fwd_T7p29, qPCR_Rvd_T7p29) RzLys_gene (qPCR_RzLys_F, qPCR_RzLys_R) E.coli K12 for T7: CysG_gene (qPCR_cysG_F, qPCR_cysG_R) idnT_gene (qPCR_cidnT_F, qPCR_cidnT_R) RrsA_gene (qPCR_rrsA_F, qPCR_rrsA_R) RFP mRFP_gene (fwd_RFP_qPCR,rvd_RFP_qPCR) |
Results: | Important: TP07 Primer does not work; T7p29 and TP01 primers work |
Gel extraction of TP01 and T7p29 PCR product
2018/06/01| Participants: | Nils O'Brien |
| Protocol: | NAME, NAME PCR from May 28th, NEB Gel extraction Kit (Monarch #T10265) | Notes: | here | Results: | here |