Team:HUST-China/Notebook

iGEM 2018 HUST-China

NoteBooks

April 1-30

Ziyang Xiao, Junhao Xiong, Jingyu Ren, Yan chen, Qian Li
Pre-experiment of electrogenesis: tested and optimized the electrogenesis condition for MFC (Microbial Fuel Cell)
May 1-31

Yiyan Yu, Ziyi Li, Junhao Xiong
Basic design of molecular experiments: design sequences, primers and the construction of Shewanella
July 1-7

Junhao Xiong, Ziqi Yin, Jiayi Liu
Preparation for following experiments including preparing LB culture medium, antibiotics and optimizing culture condition of Shewanella oneidensis MR-1.
Bo Peng
Construction of pSB1C3-RBS-mleS

Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu
Cyanobacteria Group took the responsibility of doing the Interlab. Preparation for the experiment.
July 8-21

Junhao Xiong, Ziqi Yin, Jiayi Liu
Construction of pYYDT-pflB: PCR, double enzyme digestion (with NdeI & SalI), ligation, transformation (into E.coli TOP10).

Qian Li
Construction of pSB1C3-RBS-lldp-RBS-ldhA-TT

Ziyang Xiao, Haotian Ren, Yan Chen, Hao Qiu
Finishing the cell cultures cultivation and using some machines to measure some related data.
Human Practices

The main organizers of “HUST-Cheering!” intercommunions are Shengde Deng, Jingyu Ren, Zixin Yang and Ying Xu. All members of HUST-China team helped in holding this regional jamboree. Shengde Deng, as the leader of the Human Practice, coordinated all the work, contacted company for visits and other iGEM teams for collaboration and arranged for the internal players to be responsible for the corresponding affairs. Jingyu Ren, was responsible for visit to two enterprises, Zixin Yang was responsible for the survey and editing for the tweet while Xing Xu was responsible for participating for and co-organizing the CCIC. In addition, Shengde Deng ,Zixin Xu and Xing Xu also hosted the school summer camp in order to introduce synthetic Biology and Bio battery to high school students.
Advisors

Professor Yunjun Yan
Professor Kang Ning
Dr. Yi Zhan
Thanks for their generous support and careful guidance.
Special Thanks

Especially thanks Team USTC for providing pYYDT vector and E.coli WM3064 .
Especially thanks Professor Hao Song and Dr. Feng Li for giving advice for our design.
Especially thanks Professor Chieh-Chen Huang, Dr. Shou-Chen Lo and Team NCHU_Taiwan for providing pMG105 vector and pMG105-PpckA vector.
Thanks Dr Wu Liu and Dr Qinghua Zhou for their direct guidance in experiment.
Thanks
Acknowledgments

This project was supported by Huazhong University of Science and Technology, College of Life Science and Technology and Qiming College of HUST.
Free software is offered by Mathworks.
DNA synthesis services are supported by IDT for free and by Genscript.
Primer synthesis and sequencing services are provided by Genscript, TIANYI HUIYUAN and TsingKe.