Team:Edinburgh UG/Results

Edinburgh iGEM 2018

Results

Achievements

  • We have characterised various methods of maxicell production, to optimise their manufacture for synthetic biology
  • The active time frame of maxicells was quantified, to determine their potential “lifespan”
  • We have created parts which facilitate maxicell production in any E. coli strain of your choosing
  • We have introduced a functional biosensor into the maxicell chassis, which has shown that maxicells have the potential for gene expression for at least 18 hours after their production
  • Various methods of preventing horizontal gene transfer were explored, to increase the security of maxicells as a chassis designed for environmental release
  • Triclosan resistance was introduced into a standard backbone as an alternative to antibiotic selection, which would prevent the unwanted release of antibiotic resistance genes

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