Team:Uppsala/Protocol
Worm recovery from cups
Materials:
- Cups
- Faeces
- Physiological solution (0.9% NaCl)
- Tap water
- Around 20 g of faeces are added to a cup
- The faeces are stirred with tweezers to make the sample less compact
- The cup is incubated at 28 °C for 1 week
- The cup is filled with water (200 mL)
- One half of a petri dish is placed on top. The cup with the petri dish is then turned upside down.
- The cup is left at room temperature ON, to allow the dry material to soak water and deposit on the bottom.
- The following day the cup with the petri dish are tilted to recover the liquid, which contains the worms
- The worm solution is left in the fridge for 3 hours, to allow a complete deposit of the worms.
- Most of the supernatant is removed, and the worm solution can be stored.