Team:Tokyo Tech/Parts

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Basic Parts
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Basic Parts

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Basic Parts

TokyoTech 2018 iGEM Team Basic Parts

NameTypeDescriptionDesignLength(bp)
BBa_K2729000CodingDENV1 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300

BBa_K2729001

CodingDENV2 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729002CodingDENV3 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729003CodingDENV4 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729004CodingDENV3 EHayato Ito, Kotaro Miyamoto, Hajime Fujita1479
BBa_K2729005CodingDENV4 EHayato Ito, Kotaro Miyamoto, Hajime Fujita1485
BBa_K2729006CodingEGFP_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita948
BBa_K2729007CodingDsRed_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita908
BBa_K2729008CodingYFP_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita945
BBa_K2729009CodingAmCyan_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita918
BBa_K2729010CodingDENV1 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028
BBa_K2729011CodingDENV2 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028
BBa_K2729012CodingDENV3 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2022
BBa_K2729013CodingDENV4 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028

Best Parts: EGFP-FMDV2a (BBa_K2729006)

Our team made new pseudovirus that can infect Vero cells and introduce the gene including this EGFP-FMDV2a. With the assistance of "self-cleaving" 2A peptides, fluorescence protein is produced inside of the cell and folded so that it can emit fluorescence at the end. Since EGFP contains chromophore mutations that make the protein 35 times brighter than wild-type GFP

Since EGFP is 35 times brighter than wild-type GFP and FMDV2a precisely works in our assumption, you can easily check the degree of infection by measuring fluorescence intensity.

Address

2 Chome-12-1
Ookayama, Meguro, Tokyo

Contacts

Email: igem2018tokyotech@gmail.com

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