Team:Tokyo Tech/Parts

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Basic Parts
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Basic Parts

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Basic Parts

TokyoTech 2018 iGEM Team Basic Parts

NameTypeDescriptionDesignLength(bp)
BBa_K2729000CodingDENV1 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300

BBa_K2729001

CodingDENV2 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729002CodingDENV3 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729003CodingDENV4 CHayato Ito, Kotaro Miyamoto, Hajime Fujita300
BBa_K2729004CodingDENV3 EHayato Ito, Kotaro Miyamoto, Hajime Fujita1479
BBa_K2729005CodingDENV4 EHayato Ito, Kotaro Miyamoto, Hajime Fujita1485
BBa_K2729006CodingEGFP_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita948
BBa_K2729007CodingDsRed_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita908
BBa_K2729008CodingYFP_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita945
BBa_K2729009CodingAmCyan_FMDV2aHayato Ito, Kotaro Miyamoto, Hajime Fujita918
BBa_K2729010CodingDENV1 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028
BBa_K2729011CodingDENV2 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028
BBa_K2729012CodingDENV3 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2022
BBa_K2729013CodingDENV4 AncC_prM_EHayato Ito, Kotaro Miyamoto, Hajime Fujita2028

Best Parts: EGFP-FMDV2a (BBa_K2729006)

Our team made new pseudovirus that can infect Vero cells and introduce the gene including this EGFP-FMDV2a. With the assistance of "self-cleaving" 2A peptides, EGFP is produced inside of the cell and folded so that it can emit fluorescence at the end.

Since EGFP is 35 times brighter than wild-type GFP and FMDV2a precisely works in our assumption, you can easily check the degree of infection by measuring fluorescence intensity.

Address

2 Chome-12-1
Ookayama, Meguro, Tokyo

Contacts

Email: igem2018tokyotech@gmail.com