Team:IISER-Bhopal-India
Sodales neque vitae justo sollicitudin aliquet sit amet diam curabitur sed fermentum. Methane is a Greenhouse gas associated with Global Warming,
and green methods are desired for its real-time monitoring.
Thus, we have developed the prototype of a robust field-applicable methane biosensor,
MethNote. We found an enzyme-complex methane monooxygenase(MMO) from Methylococcus capsulatus,
a methanotrophic bacterium, that converts methane to methanol. We expressed soluble-MMO in the methylotrophic yeast,
Pichia pastoris, which harbors a plasmid expressing the reporter gene under a methanol inducible promoter AOX.
Thus, linking methane uptake to a reporter gene expression generates the proposed methane biosensor. The inclusion of
sMMO pathway was also checked by metabolic modeling. The constructed part will be a useful contribution to the iGEM repository.
A commercial design of MethNote will find widespread applications in environmental monitoring of methane. In future studies,
we also anticipate an additional application of Mut- strain of P. pastoris expressing sMMO in biofuel production through
methanol sequestration.
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