Team:BCU/Description
Description
Nicotine (1-methyl-2-[3-pyridyl-pyrrolidine], C10H14N2), playing a major role in tobaccos, is an indispensable factor in producing cigarettes. Smoking addiction and is well known addiction due to the intake nicotine contained in cigarettes. Intake of nicotine could cause serious problems in human body, because it easily crosses the blood-brain barrier and biological membranes. In another case, as customers demand for cigarettes, massive amount of tobacco were being processed, this caused tobacco waste. Aminobiphenyl, naphthylamine, and benzo(a)- pyrene, were all examples of pollution that created and entered in the environment. Moreover, nicotine and many alkaloids are all not recyclable, so when those are being discharged, they brings a big impact on environment and causes pollution.
配个图片，以上文字主要说尼古丁对人体和环境的污染。
2. 2.Recently, there were a lot more nicotine-degrading bacteria researched being reported, and the degradation pathways of nicotine was more and more clear. The degradation of nicotine through the pyrrolidine pathway by Pseudomonas is extensive and well studied.
图片添加：尼古丁酶催化降解途径的图，要是清楚的话可以通过chemdraw画一个
In our study, the first three gene_____,_______and______(增加三个基因的名字和基因序列号) are crucial for nicotine degradation, so we have construct a pathway to express the three gene in top10.
图片添加：载体构建图和实验流程图
基因路线图中的汉语的翻译分别是
Nicotine degradation
Gene cloning
Pathway construction
Vector construction
Protein expression
Screening
Nicotine catalysis
以上除了图片是所有项目介绍内容
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