Team:OUC-China/Experiments

Team OUC-China: Main

Experiments

This year, our project can be divided into four systems. Between the different systems, there are different protocols. And we have sorted out these experimentals in detail.

1.Elementary Operation

（1）Conservation
（2）Gel Electrophoresis
（3）Gel Extraction
（4）Inoculation
（5）Ligation system
（6）medium
（7）Plasmid extraction
（8）Polymerase Chain Reaction
（9）Restriction Enzyme Digestion
（10）Sterilization
（11）Transformation of Escherichia coli

2.Systems Detection

（1）Assay of Gene Expression by Flow Cytometry
（2）Fluorescence measurement
（3）miniToe Polycistron Fluorescence measurement
（4）The application of miniToe——Regulation of motA