Team:TecCEM/Hardware

Hardware

Project/ Hardware

Index

• TAC-O/BOB
• Automation System
• Sketches
• Design
• Arduino's code
• Video

The hardware developed for this year is an automated system aimed to help the scientific community improve and develop new pharmaceutical products by facilitating the analysis of new drugs’ effects on cell cultures. This hardware is conformed by two main parts; a small glass flask named BOB and the automation system.

TAC-O/BOB

As mentioned previously, BOB is a small tempered glass flask designed to test molecule’s effect on cell cultures. Due to the properties of tempered glass, it can be depyrogenated which will allow us to reuse this flask more than once. This present diverse advantages in which they are included to be eco friendly, to be cheaper and to be easier to wash.

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In the front side there is a small hole which will allow us to introduce the new cell culture medium whenever the old one is ready to be changed. Likewise, there is a elbow at the bottom which will help us to get rid of the old medium. Connected to this elbow we have a 3-way stopcock which will open using a servo-motor. We will talk more about it in the Automation System section.

To help BoB fulfill its function, we use small 5mm septas (white pieces in Figure 1) which allow us to introduce the new cell culture medium inside it via a syringe and also prevent the medium from flowing outside BoB.

Inside BoB we can find a PDMS membrane, in which the cells will rest, that is suspended ⅓ the flasks height over the bottom. The reason of the ⅓ is because we need the culture to have certain air space for correct cells’ oxygen diffusion. It was reported that optimal level of the medium is 0.2 cm, that is the equivalent to a volume of 0.2 mL/cm2 [1]. The membranes recipe is shown in Figure 2.

To keep the PDMS membrane suspended, we use orthodontic archwire to supports the membrane from below. This archwire is then attached to BoB’s lid with the help of two holes and 2 septas as seen in Figure 1.

The third hole on Bob’s lid is used to introduce the cells, using a syringe, inside the flask; a septa is also used in this hole so that the entire system is closed.

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To ensure the correct system’s behavior we created a combined base for Bob and the servo-motor that will open the 3-way stopcock. BoB’s part of the base has an inclination of 7.3 degrees to allow the medium to flow outside the flask, it also permits us to put a color sensor below the flask to measure the medium’s color. This part of the base also grabs the 3-way stopcock to prevent the servo from twisting it.

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Automation System

For the correct use of the system, the input and output of the culture medium is required; these inputs and outputs are automatized to save time, reagents and preserve the sterile conditions of the environment thanks to the low interaction with humans. Using standardized times, volumes and parameters we implemented the automation of the system, resulting in a device that operates specifically for these objectives.

To make the automation system we used the following material:

• Arduino nano, bipolar stepper motor
• 3 way stopcock
• 3 piece syringe
• a servo motor, nodemcu board, dc driver, 5 Volts regulator (7805), pumping mechanism made of acrylic and iron.

The Arduino Nano is the main device, providing the system with enough processing power to take the decisions. This board is the one in charge of processing the color sensor input, controlling the stepper motor and the servo motor, and dispatching the data from the sensor to the Nodemcu board. A friendly diagram of the electronics involved is shown below.

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The Nodemcu take input from the arduino and sends the data to a Pyhton’s Flask server hosted in Heroku. This data can then be visualized in an web application built in React JS-Redux. This application is further explained in the software section.

The pumping system is composed by 3 main elements. The first one is the bipolar stepper motor which provides enough torque and precision and is used to inject the medium. The second part is the mechanism itself, shown in the below figure, which is made out of the acrylic base, the syringe and the endless screw. The last element is the servo motor, utilized to control the output of the medium.

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The sensing of the system is made with the help of a TCS3200 color sensor. This sensor returns an RGB code which will then be transformed into a single value using the next function.

$Value\; =\; Rb\; *\; 64\; +\; Gb\; *\; 8\; +\; Bb$
Rb = R value in binary
Gb = G value in binary
Bb = B value in binary

The medium change sequence was programmed for it to be emitted when ever the sensor recognizes an orangish tone from the medium. When this happens, the arduino executes this sequence and the change occurs.

The sequence follows the next order:

1. Open the control valve.
2. Introduce cleaning medium (2ml).
3. Close the outlet valve.
4. Introduce 6.5ml of medium to fill the flask again.

In conclusion, all hardware built during this time works perfectly allowing us to fully automate the change and sensing of the medium while providing this data to a server. The advantages of this is enormous if applied correctly, in the below Improvement section we talk more about this topic and the future of this hardware.

Sketches

Design

Arduino's code

Github BoB Automation

Video

Improvement

As part of the improvements, we will build a fully functional glass incubator. To replicate the same behavior of an already existing one, we will control temperature and CO2 flux and support an air filtration system. We will also integrate a microscope to watch the cell culture.

To control the temperature, we will design a close loop control system, using a NTC10KB3950K sensor to measure this variable as close to the culture as possible. In the same way, we will design a close loop control for the CO2 flux using a ASINPPOKAL25818 sensor to measure this variable.

The purpose of this incubator is to build a single interaction cell culture system that can be place anywhere in the lab. An image can be seen below showing a mock of the incubators size .

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