Template:Groningen/Attributions

Attributions

“You give a man a fish and you feed him for a day. You teach him to fish and you give him an occupation that will feed him for a lifetime.” (Chinese proverb)

We, the University of Groningen iGEM team, were responsible for almost all aspects of StyGreen. From coming up with the concept, running a fully-functioning interdisciplinary team, finding supporters to finance our quest and executing, designing and running the wet- and dry lab work to name some. However, these past 6 months we have aimed to push ourselves beyond our personal limits. This page is dedicated to all those who helped us along the way. We want to thank you for your time, knowledge, patience, support and for believing in us!

• PI's
  Jan Kok

  Dirk-Jan Scheffers
• Instructors
  Andreas Milias

  Linda Dijkshoorn

  Agnieszka Wegrzyn

  Sergey Lunev
• General Support
  Lisbeth Ravnkilde Kjolbye: for providing us with many contacts to get our project off the ground

  Thanks to Matthijs’ personnel pass for free coffee

  Sonja Billerbeck: advice about the iGEM competition in general

  Siger Holsappel: general support in logistics

  Ger Telkamp: for providing us with lab-equipment from the practicum pool

  Manon Dusseljee: for financial administrative support

  Hannah Dosenko: for helping us with our ....

  Winand Slingenbergh and Fablab Groningen: for helping us with our 3D print designs
• Fundraising help and advice
  Chienes Metus: For aiding us in our financial planning and taking care of all of our bills

  Mareike Schmidt: For giving advice on organizing our fundraising barbecue

  Derk Bosker: For guidance in the crowd-funding page
• Lab Support
  Tau Jang: for providing us with A. Thaliana for extracting the PAL2 and general lab advice

  Jeroen Nijland: for guiding us with CRISPR-Cas9 and providing us with an extra lab and protocols and giving access to HPLC system

  Arnold Driessen: for advice about the use of CRISPR-Cas9 and evolution experiments in our project and lab access

  Jan Kiel: for helping us setting up our lab and providing us with cellulose

  Wiktor Szymanski: for advice about phosphorylation of cellulose and for allowing us to use space and materials in their laboratory

  Dirk Jan Slotboom: for providing us with access to a plate reader for the Interlab study

  Aditya Iyer: for help in the Interlab plate-reader experiments and supply of lab materials and performing EGII Western blot

  Hemant Kumar: for guidance in the cellulase activity assays & advise how to improve the HPLC protocol for the Bordeaux collaboration

  Alisa Garaeva: for providing us with a plasmid for our collaboration with iGEM Leiden

  Amanda van Tilburg: for delivery of primers and donation of BL21(DE3) strain

  Buu Minh Tran: for providing us with a plasmid for our collaboration with iGEM Leiden

  Vakhil Takhaveev: for being so kind to already transform these plasmids in E. coli for us

  Jakob Viel: for helping us find -80 freezer space, and locating some chemicals

  Marisya Majsnerowska: for providing us with materials to visualise our His-tagged proteins on Western blots

  Andre Boltjes: for feedback on the styrene extraction protocol

  Ruben Maaskant: for helping with the GC-MS and GC-FID methods

  Arjen Krikken: for providing strains, plasmids, and some restriction enzymes

  Huala Wu: for providing YPD Zeocin plates

  Anna Lauxen: for instructions on the Tecan Geneos platereader

  Niels de Kok: for providing us with E. coli DH5a and BL21 (DE3)

  Marten Exterkate: for cloning advice and helping with EGII purification

  László Mózsik: for providing a kanamycin stock and general lab advice

  Chris Williams: for providing general tips and zeocin

  Jhonatan Hernandez-Valdes: for instructions on the Thermo-Scientific Varioskan LUX platereader

  Luiza Morawska: for instructions on the Olympus DeltaVision fluorescence microscope

  Johan Kemmink: for helping us with phosphorus NMR
• Lab design Support
  Bert Poolman: for giving us advice on how to export styrene from the cell

  Jan Willem Meints: for helping us develop a HPLC quantification method for styrene

  Marco Fraaije: for advice about a cellulase-activity assay

  Dick Janssen: For advice on experimental design of the enzymatic cascade
• Personal development and coaching
• (Integrated) Human Practices and Public Engagement Support
  Karin Ree: for giving us tips on who to contact

  Tjerk Douwma: for helping us doing carbon footprint analysis (Life Cycle Analysis)

  Katja Loos: for advice on biodegradable plastics

  Francesco Picchioni: for advise on what plastic to go for

  Martin van Dord: for advice about plastics and rubbers (NRK)

  Pieter Imhof: for advice about the production of biochemicals (BioBTX)

  Machiel van Steenis (Program manager Innovative Biomass Applications): for advice about the application of biomass-based technologies

  Gert-Jan Euverink: for advice about the use of sewage material as a source of our cellulose and technical improvements for our yeast strain

  Frans Timmermans: for helping us extrapolate our project to alternative biomass sources (algae as cellulose source)

  Andre Fay: for sharing his expertise in the field of biomass

  Korienke Smit and Niek Savelkoul: for coaching us about integrated human practices (RIVM)

  ScienceLinx: for helping us with education and public engagement

  Scholierenacademie: for giving us the opportunity to educate primary school students about … Jens knows this

  Paddepoel: for giving us a stage for our outreach event to educate the general population on science and our project in general

  Girbe Buist: for supplying us with materials for our outreach event in Paddepoel

  Rene Fransen: for …

  Jeroen Tieleman (Bioclear Earth): for giving us tips on the resource of biomass
• Outreach and Social Media
  Daniel Oostenbrink: for helping us fine-tune our animation’s script

  Joris-Jan Coenraads: for lending us his microphone to record our animation
• Drylab Support
  Agnieszka Wegrzyn: for general modelling advice and advice on flux based modelling

  Matthias Heinemann: for giving us advice on modelling our project using Flux balance Analysis

  Siewert-Jan Marrink: for discussing modelling approaches with us

  Pim Frederix: for giving us a group workshop on Molecular Dynamics

  Paolo Telles de Souza: for helping to setup the cellulose binding molecular dynamics model