Team:WLC-Milwaukee/Notebook

PLACEHOLDER

WLC iGEM 2018 | Timeline

Project Timeline

Lorem ipsum dolor sit amet, consectetur adipiscing elit,Lorem ipsum dolor sit amet, consectetur adipiscing elit Lorem ipsum dolor sit amet, consectetur adipiscing elit,Lorem ipsum dolor sit amet, consectetur adipiscing elit



Project Decisions!

February 2018

Parts

Based on Judge feedback and our desire to bring the 2017 WLC-Milwaukee project to fruition, we decided to continue our 2017 project detecting E. coli in contaminated water supplies using Lambda Phage tail proteins. We began laying out the ways we would improve our past project and complete the areas where it had fallen short and been unsuccessful in 2017.

Began Cloning parts into pTrc99a

March 2018

Parts

With the help of our advisor, we designed primers and began the frustrating process of amplifying and cloning our parts from the pETail4 plasmid (containing the Lambda Phage tail protein genes) into pTrc99a in order to control protein expression levels using the pTrc99a IPTG inducible trc-promoter (also called Ptac).

MUBRC

April 2018

Parts

A number of our team members participated in MUBRC (The Milwaukee Undergraduate Biological Research Conference) by volunteering and helping facilitate setup for this event while Jack presented a poster of our past work and hopeful project improvements in order to gain feedback from other undergraduate researchers and PIs in attendance.

Started Human Practices Investigation

June 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Organization Outreach Day

August 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Confirmed Clones in pTrc99a

September 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Science Exploration Day

September 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Protein Purification and Analysis

October 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

System Prototyping and Continued Testing

October 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Young Women in Science Program

March 3rd 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Planned Outreach Events

April 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

New Cloning Primers

May 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Jack Tours Cornell Lab Spaces

August 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Spoke with Experts to Analyze our System

September 2018

Parts

Modified our project design, usability, and scope of its application based on feedback and re-analysis of our system.

Tested HRP and Substrates based on new system goals

September 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Obtained clones in pSB1C3

October 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Visited MMSD treatment plant

October 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in

Submitted Parts and finished the Wiki!

October 2018

Parts

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiucol-smod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in